Parasitological detection of Trypanosoma brucei gambiense in serologically negative sleeping-sickness suspects from north-western Uganda

Simple item page
dc.contributor.authorEnyaru, J. C. K.
dc.contributor.authorMatovu, E.
dc.contributor.authorAkol, M.
dc.contributor.authorSebikali, C.
dc.contributor.authorKyambadde, J.
dc.contributor.authorSchmidt, C.
dc.contributor.authorBrun, R.
dc.contributor.authorKaminsky, R.
dc.contributor.authorOgwal, L. M.
dc.contributor.authorKansiime, F.
dc.date.accessioned2022-05-02T22:57:41Z
dc.date.available2022-05-02T22:57:41Z
dc.date.issued2016
dc.description.abstractForty-five parasitologically confirmed cases of sleeping sickness were diagnosed in north-western Uganda using a combination of two or three techniques. Forty of the cases were positive by the card agglutination test for trypanosomiasis (CATT), four were negative and one was not screened by the CATT. Trypanosomes isolated from the four CATT-negative but parasitologically positive cases were propagated for detailed biochemical genetic analysis. The aim was to demonstrate whether these four stocks lacked the LiTat 1.3 gene which encodes the antigen on which the CATT is based. All the DNA extracts isolated from these CATT-negative stocks and from six CATT-positive stocks of Trypanosoma brucei gambiense were targeted for amplification by the three variable-surface-glycoprotein genes thought to be ubiquitous in T. b. gambiense. The LiTat 1.3 gene was shown to be present in all 10 stocks. Trypanosome carriers may be CATT -negative because the CATT is not sensitive enough, because their parasites lack the LiT at 1.3 gene, or because their parasites have this gene but do not express it. The four sleeping-sickness cases who gave negative CATT results in the present study have very important implications in the diagnosis of T. b. gambiense infections using the CATT. Following treatment of the CATT -positive cases, the CA TT -negative carriers of the trypanosomes remain as human reservoir hosts for continuous infection of the population. Because CATT-negative individuals are rarely examined further, the general prevalence of parasitologically positive but CATT-negative cases is unclear. This study demonstrates the value of co-ordinated use of serological and parasitological techniques in the diagnosis of Gambian sleeping sickness.en_US
dc.identifier.citationJ. C. K. Enyaru, E. Matovu, M. Akol, C. Sebikali, J. Kyambadde, C. Schmidt, R. Brun, R. Kaminsky, L. M. Ogwal & F. Kansiime (1998) Parasitological detection of Trypanosoma brucei gambiense in serologically negative sleeping-sickness suspects from north-western Uganda, Annals of Tropical Medicine & Parasitology, 92:8, 845-850, DOI: 10.1080/00034983.1998.11813349en_US
dc.identifier.other10.1080/00034983.1998.11813349
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3180
dc.language.isoenen_US
dc.publisherAnnals of Tropical Medicine & Parasitologyen_US
dc.subjectParasitological detectionen_US
dc.subjectTrypanosoma brucei gambienseen_US
dc.subjectSerologically negative sleepingsickness suspectsen_US
dc.subjectNorth-western Ugandaen_US
dc.titleParasitological detection of Trypanosoma brucei gambiense in serologically negative sleeping-sickness suspects from north-western Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Parasitological detection of Trypanosoma brucei.pdf
Size:
566.78 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences