Correlation between Presence of Trypanosoma cruzi DNA in Heart Tissue of Baboons and Cynomolgus Monkeys, and Lymphocytic Myocarditis

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dc.contributor.authorMubiru, James N.
dc.contributor.authorYang, Alice
dc.contributor.authorDick, Edward J.
dc.contributor.authorOwston, Michael
dc.contributor.authorSharp, Mark
dc.contributor.authorVandeBerg, Jane F.
dc.contributor.authorShade, Robert E.
dc.contributor.authorVandeBerg, John L.
dc.date.accessioned2023-06-12T21:02:33Z
dc.date.available2023-06-12T21:02:33Z
dc.date.issued2014
dc.description.abstractTrypanosoma cruzi, the causative agent of Chagas’ disease, preferentially infects cardiac and digestive tissues. Baboons living in Texas (Papio hamadryas) and cynomolgus monkeys (Macaca fascicularis) have been reported to be infected naturally with T. cruzi. In this study, we retrospectively reviewed cases of animals that were diagnosed with lymphocytic myocarditis and used a polymerase chain reaction (PCR)-based method (S36/S35 primer set) to amplify T. cruzi DNA from archived frozen and formalin-fixed paraffin-embedded (FFPE) cardiac tissues. We show that the PCR method is applicable in archived frozen and FFPE tissues and the sensitivity is in the femtogram range. A positive correlation between PCR positivity and lymphocytic myocarditis in both baboons and cynomolgus monkeys is shown. We also show epicarditis as a common finding in animals infected with T. cruzi.en_US
dc.identifier.citationMubiru, J. N., Yang, A., Dick Jr, E. J., Owston, M., Sharp, R. M., VandeBerg, J. F., ... & VandeBerg, J. L. (2014). Correlation between presence of Trypanosoma cruzi DNA in heart tissue of baboons and cynomolgus monkeys, and lymphocytic myocarditis The American Journal of Tropical Medicine and Hygiene , 90(4), 627.en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8909
dc.language.isoenen_US
dc.publisherThe American Journal of Tropical Medicine and Hygieneen_US
dc.subjectTrypanosoma cruzi DNAen_US
dc.subjectHeart Tissueen_US
dc.subjectBaboons and Cynomolgus Monkeysen_US
dc.subjectLymphocytic Myocarditisen_US
dc.titleCorrelation between Presence of Trypanosoma cruzi DNA in Heart Tissue of Baboons and Cynomolgus Monkeys, and Lymphocytic Myocarditisen_US
dc.typeArticleen_US
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