Single-Cell Level Response of HIV-Specific and Cytomegalovirus-Specific CD4 T Cells Correlate With Viral Control in Chronic HIV-1 Subtype A Infection

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dc.contributor.authorEller, Michael A.
dc.contributor.authorEller, Leigh Anne
dc.contributor.authorRatto-Kim, Silvia
dc.contributor.authorOuma, Benson J.
dc.contributor.authorLo, Vicky
dc.contributor.authorSouza, Mark de
dc.contributor.authorGuwatudde, David
dc.contributor.authorNails, Barbara
dc.contributor.authorMichael, Nelson L.
dc.contributor.authorWabwire-Mangen, Fred
dc.contributor.authorRobb, Merlin L.
dc.contributor.authorMarovich, Mary A.
dc.contributor.authorSandberg, Johan K.
dc.contributor.authorCurrier, Jeffrey R.
dc.date.accessioned2022-04-30T08:08:34Z
dc.date.available2022-04-30T08:08:34Z
dc.date.issued2012
dc.description.abstractHIV-1 subtype A is the second most prevalent subtype globally and is associated with reduced viral load, higher CD4 absolute counts, and slower disease progression. To study the possible role of T cells associated with better outcome, we examined CD4 and CD8 T-cell responses against HIV-1 and cytomegalovirus (CMV) in Ugandans infected with subtype A HIV-1. Methods: T-cell responses were investigated using flow cytometry and novel subtype A variant inclusive peptide (VIP) sets designed for this evaluation. CD4 T-cell responses focused primarily on Gag, whereas CD8 T-cell responses were broadly directed against Gag, gp41, and Nef VIP sets. CD4 T cells primarily responded with interferon (IFN)-g, whereas CD8 cells were more diverse with degranulation (CD107a), IFN-g, and macrophage inflammatory protein (MIP)-1b production. Results: No relationship was observed between CD8 T-cell responses and the HIV-1 load. Similarly, the frequency of CD4 T cells responding to these antigens did not associate with viral control. However, in CD4 T cells responding against Gag or CMV, the IFN-g intensity, indicative of the production at the singlecell level, was inversely proportional to viral load. No significant relationship was found between T-cell effector/memory phenotype and viral control. Conclusions: The per cell production of IFN-g in CD4 T cells responding to HIV-1 or CMV correlated with viral control in chronic HIV-1 subtype A infection. These data suggest that quantitative aspects at the single-cell level may be more important than the frequency of antigen-specific CD4 T cells in HIV-1 subtype A infection control.en_US
dc.identifier.citationEller, M. A., Eller, L. A., Ratto-Kim, S., Ouma, B. J., Lo, V., de Souza, M., ... & Currier, J. R. (2012). Single-cell level response of HIV-specific and cytomegalovirus-specific CD4 T cells correlate with viral control in chronic HIV-1 subtype A infection. JAIDS Journal of Acquired Immune Deficiency Syndromes, 61(1), 9-18.en_US
dc.identifier.urihttps://journals.lww.com/jaids/fulltext/2012/09010/Single_Cell_Level_Response_of_HIV_Specific_and.2.aspx
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3031
dc.language.isoenen_US
dc.publisherJAIDS Journal of Acquired Immune Deficiency Syndromesen_US
dc.subjectT cellsen_US
dc.subjectHIV-1en_US
dc.subjectAIDSen_US
dc.subjectViral infectionsen_US
dc.subjectMemoryen_US
dc.titleSingle-Cell Level Response of HIV-Specific and Cytomegalovirus-Specific CD4 T Cells Correlate With Viral Control in Chronic HIV-1 Subtype A Infectionen_US
dc.typeArticleen_US
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