Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola)
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Asian Pacific journal of tropical medicine
Abstract
To determine the phytochemical composition, antioxidant and anticancer activities of
ethanolic and water leaves extracts of Annona muricata (A. muricata) from the Eastern Uganda.
Methods: Phytochemical screening was conducted using standard qualitative methods
and a Chi-square goodness of fit test was used to assign the relative abundance of the
different phytochemicals. The antioxidant activity was determined using the 2, 2-diphenyl2-picrylhydrazyl and reducing power methods whereas the in vitro anticancer activity was
determined using three different cell lines.
Results: Phytochemical screening of the extracts revealed that they were rich in secondary
class metabolite compounds such as alkaloids, saponins, terpenoids, flavonoids, coumarins and
lactones, anthraquinones, tannins, cardiac glycosides, phenols and phytosterols. Total phenolics
in the water extract were (683.69依0.09) µg/mL gallic acid equivalents (GAE) while it was (372.92
依0.15) µg/mL GAE in the ethanolic extract. The reducing power was 216.41 µg/mL in the water
extract and 470.51 µg/mL GAE in the ethanolic extract. In vitro antioxidant activity IC50 was 2.0456
mg/mL and 0.9077 mg/mL for ethanolic and water leaves extracts of A. muricata respectively. The
ethanolic leaves extract was found to be selectively cytotoxic in vitro to tumor cell lines (EACC,
MDA and SKBR3) with IC50 values of 335.85 µg/mL, 248.77 µg/mL, 202.33 µg/mL respectively, while
it had no cytotoxic effect on normal spleen cells. The data also showed that water leaves extract of
A. muricata had no anticancer effect at all tested concentrations.
Conclusions: The results showed that A. muricata was a promising new antioxidant and
anticancer agent.
Description
Keywords
Annona muricata, Phytochemical screening, Antioxidant, Anticancer
Citation
Gavamukulya, Y., Abou-Elella, F., Wamunyokoli, F., & AEl-Shemy, H. (2014). Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pacific journal of tropical medicine, 7, S355-S363. doi: 10.1016/S1995-7645(14)60258-3