Analysis of Prostate-Specific Antigen Transcripts in Chimpanzees, Cynomolgus Monkeys, Baboons, and African Green Monkeys

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dc.contributor.authorMubiru, James N.
dc.contributor.authorYang, Alice S.
dc.contributor.authorOlsen, Christian
dc.contributor.authorNayak, Sudhir
dc.contributor.authorLivi, Carolina B.
dc.contributor.authorDick Jr., Edward J.
dc.contributor.authorOwston, Michael
dc.contributor.authorGarcia-Forey, Magdalena
dc.contributor.authorShade, Robert E.
dc.contributor.authorRogers, Jeffrey
dc.date.accessioned2023-06-01T16:28:02Z
dc.date.available2023-06-01T16:28:02Z
dc.date.issued2014
dc.description.abstractThe function of prostate-specific antigen (PSA) is to liquefy the semen coagulum so that the released sperm can fuse with the ovum. Fifteen spliced variants of the PSA gene have been reported in humans, but little is known about alternative splicing in nonhuman primates. Positive selection has been reported in sex- and reproductive-related genes from sea urchins to Drosophila to humans; however, there are few studies of adaptive evolution of the PSA gene. Here, using polymerase chain reaction (PCR) product cloning and sequencing, we study PSA transcript variant heterogeneity in the prostates of chimpanzees (Pan troglodytes), cynomolgus monkeys (Macaca fascicularis), baboons (Papio hamadryas anubis), and African green monkeys (Chlorocebus aethiops). Six PSA variants were identified in the chimpanzee prostate, but only two variants were found in cynomolgus monkeys, baboons, and African green monkeys. In the chimpanzee the full-length transcript is expressed at the same magnitude as the transcripts that retain intron 3. We have found previously unidentified splice variants of the PSA gene, some of which might be linked to disease conditions. Selection on the PSA gene was studied in 11 primate species by computational methods using the sequences reported here for African green monkey, cynomolgus monkey, baboon, and chimpanzee and other sequences available in public databases. A codon-based analysis (dN/dS) of the PSA gene identified potential adaptive evolution at five residue sites (Arg45, Lys70, Gln144, Pro189, and Thr203).en_US
dc.identifier.citationMubiru JN, Yang AS, Olsen C, Nayak S, Livi CB, et al. (2014) Analysis of Prostate-Specific Antigen Transcripts in Chimpanzees, Cynomolgus Monkeys, Baboons, and African Green Monkeys. PLoS ONE 9(4): e94522. doi:10.1371/journal.pone.0094522en_US
dc.identifier.other10.1371/journal.pone.0094522
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8865
dc.language.isoenen_US
dc.publisherPLoS Oneen_US
dc.subjectProstateen_US
dc.subjectTranscriptsen_US
dc.subjectChimpanzeesen_US
dc.subjectCynomolgus Monkeysen_US
dc.subjectBaboonsen_US
dc.subjectAfrican Green Monkeysen_US
dc.titleAnalysis of Prostate-Specific Antigen Transcripts in Chimpanzees, Cynomolgus Monkeys, Baboons, and African Green Monkeysen_US
dc.typeArticleen_US
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