Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)

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dc.contributor.authorFagre, Anna C.
dc.contributor.authorLewis, Juliette
dc.contributor.authorMiller, Megan R.
dc.contributor.authorMossel, Eric C.
dc.contributor.authorLutwama, Julius J.
dc.contributor.authorNyakarahuka, Luke
dc.contributor.authorNakayiki, Teddy
dc.contributor.authorKityo, Robert
dc.contributor.authorNalikka, Betty
dc.contributor.authorTowner, Jonathan S.
dc.contributor.authorAmman, Brian R.
dc.contributor.authorSealy, Tara K.
dc.contributor.authorFoy, Brian
dc.contributor.authorSchountz, Tony
dc.contributor.authorAnderson, John
dc.contributor.authorKading, Rebekah C.
dc.date.accessioned2022-09-10T10:30:34Z
dc.date.available2022-09-10T10:30:34Z
dc.date.issued2021
dc.description.abstractSerological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus RNA (sfRNA) of the 3′ untranslated region (UTR), which persists in tissues due to XRN-1 stalling during RNA decay. We amplified ZIKV sfRNA but not NS5 from three experimentally-infected Jamaican fruit bats, supporting the hypothesis of sfRNA tissue persistence. Applying this approach to 198 field samples from Uganda, we confirmed presence of ZIKV sfRNA, but not NS5, in four bats representing three species: Eidolon helvum (n = 2), Epomophorus labiatus (n = 1), and Rousettus aegyptiacus (n = 1). Amplified sequence was most closely related to Asian lineage ZIKV. Our results support the use of sfRNA as a means of identifying previous flavivirus infection and describe the first detection of ZIKV RNA in East African bats.en_US
dc.identifier.citationFagre, A. C., Lewis, J., Miller, M. R., Mossel, E. C., Lutwama, J. J., Nyakarahuka, L., ... & Kading, R. C. (2021). Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae). Scientific reports, 11(1), 1-8. https://doi.org/10.1038/s41598-021-87816-5en_US
dc.identifier.urihttps://doi.org/10.1038/s41598-021-87816-5
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4669
dc.language.isoenen_US
dc.publisherScientific reportsen_US
dc.subjectSubgenomic flavivirus RNA (sfRNA)en_US
dc.subjectAsian lineage Zika virusen_US
dc.subjectUgandan bats (family Pteropodidae)en_US
dc.titleSubgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)en_US
dc.typeArticleen_US
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