Galinsoga parviflora restored associated motor coordination through increased linear distribution of Purkinje Cells in mercury chloride-induced toxicity of mice’s cerebellum
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
African Journal of Cellular Pathology
Abstract
Mercury (Hg) is a poisonous substance associated with diseases, such as ataxia and Joubert
syndrome. Therefore, it is important to find a way to disrupt the process of Hg poisoning in the
cerebellum, by exploring the medicinal value of local herb such as Galinsoga parviflora (GP). This study
examined the effects of aqueous leaf extract of GP in HgCl2-induced cerebellar toxicity in adult male
mice. Twenty-five adult male mice of an average weight of 25 g were randomly divided into 5 groups
(n=5). Animals in Group I received oral administration of 2 ml/kg distilled water for 6 weeks, Group II
received 2.3 mg/kg of HgCl2 for 3 weeks, Group III received 2.3 mg/kg of HgCl2 for the first 3 weeks
followed by oral administration of 800mg/kg of GP extract for the next 3 weeks, Group IV received
800mg/kg of GP extract for the first 3 weeks followed by 2.3 mg/kg of HgCl2 for the next three weeks,
and Group V received 2.3 mg/kg of HgCl2 and 800 mg/kg of GP extract concurrently for three weeks. The
test animals were subjected to beam walking tests during the experiment period, followed by
euthanasia, perfusion fixation, and tissue sample collection for histological and histochemical analysis.
Treatment with the extract of GP showed varying degrees of regeneration in the cerebellar Purkinje
cells and myelin sheath of mice in HgCl2 induced toxicity, with corresponding improvements in balance
and posture. Cerebellar HgCl2 exposure in the present study was neurotoxic; however, treatment with
GP was of therapeutic value.
Description
Keywords
Mercury chloride, Cerebellum, Galinsoga parviflora
Citation
Ayuba, J. T., Okesina, A. A., Usman, I. M., Ajenikoko, M. K., Pius, T., Kusiima, N., ... & Edgar, M. F. (2022). Galinsoga parviflora restored associated motor coordination through increased linear distribution of Purkinje Cells in mercury chloride-induced toxicity of mices cerebellum. African Journal of Cellular Pathology, 14(1), 1-8. DOI: 10.5897/AJCPath2022.0035