Antiviral Innate Immune Activation in HIV-Infected Adults Negatively Affects H1/IC31-Induced Vaccine-Specific Memory CD4 T Cells

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dc.contributor.authorLenz, Nicole
dc.contributor.authorKagina, Benjamin M.
dc.contributor.authorLukindo, Tedson
dc.contributor.authorMpina, Maxmillian
dc.contributor.authorDaubenberger, Claudia A.
dc.date.accessioned2023-02-13T19:09:51Z
dc.date.available2023-02-13T19:09:51Z
dc.date.issued2015
dc.description.abstractTuberculosis (TB) remains a global health problem, with vaccination being a necessary strategy for disease containment and elimination. A TB vaccine should be safe and immunogenic as well as efficacious in all affected populations, including HIV-infected individuals. We investigated the induction and maintenance of vaccine-induced memory CD4+ T cells following vaccination with the subunit vaccine H1/IC31. H1/IC31 was inoculated twice on study days 0 and 56 among HIV-infected adults with CD4+ lymphocyte counts of >350 cells/mm3. Whole venous blood stimulation was conducted with the H1 protein, and memory CD4+ T cells were analyzed using intracellular cytokine staining and polychromatic flow cytometry. We identified high responders, intermediate responders, and nonresponders based on detection of interleukin-2 (IL-2), tumor necrosis factor alpha (TNF-α), and gamma interferon (IFN-γ) expressing central (TCM) and effector memory CD4+ T cells (TEM) 182 days after the first immunization. Amplicon-based transcript quantification using next-generation sequencing was performed to identify differentially expressed genes that correlated with vaccine-induced immune responses. Genes implicated in resolution of inflammation discriminated the responders from the nonresponders 3 days after the first inoculation. The volunteers with higher expression levels of genes involved in antiviral innate immunity at baseline showed impaired H1-specific TCM and TEM maintenance 6 months after vaccination. Our study showed that in HIV-infected volunteers, expression levels of genes involved in the antiviral innate immune response affected long-term maintenance of H1/IC31 vaccine-induced cellular immunity.en_US
dc.identifier.citationLenz, N., Schindler, T., Kagina, B. M., Zhang, J. D., Lukindo, T., Mpina, M., ... & Daubenberger, C. A. (2015). Antiviral innate immune activation in HIV-infected adults negatively affects H1/IC31-induced vaccine-specific memory CD4+ T cells. Clinical and vaccine immunology, 22(7), 688-696.en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7787
dc.language.isoenen_US
dc.publisherClinical and vaccine immunologyen_US
dc.subjectTuberculosisen_US
dc.subjectCD4+en_US
dc.subjectAntiviralen_US
dc.subjectHIV-Infected Adulten_US
dc.titleAntiviral Innate Immune Activation in HIV-Infected Adults Negatively Affects H1/IC31-Induced Vaccine-Specific Memory CD4 T Cellsen_US
dc.typeArticleen_US
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Antiviral Innate Immune Activation in HIV-Infected Adults Negatively Affects H1/IC31-Induced Vaccine-Specific Memory CD4+ T Cells
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