Evaluation of Pneumococcal Load in Blood by Polymerase Chain Reaction for the Diagnosis of Pneumococcal Pneumonia in Young Children in the PERCH Study

dc.contributor.authorDeloria Knoll, Maria
dc.contributor.authorMorpeth, Susan C.
dc.contributor.authorScott, J. Anthony G.
dc.contributor.authorWatson, Nora L.
dc.contributor.authorPark, Daniel E.
dc.contributor.authorBaggett, Henry C.
dc.contributor.authorBrooks, W. Abdullah
dc.contributor.authorFeikin, Daniel R.
dc.contributor.authorHammitt, Laura L.
dc.contributor.authorHowie, Stephen R. C.
dc.contributor.authorKotloff, Karen L.
dc.contributor.authorLevine, Orin S.
dc.contributor.authorO’Brien, Katherine L.
dc.contributor.authorThea, Donald M.
dc.contributor.authorAhmed, Dilruba
dc.contributor.authorAntonio, Martin
dc.contributor.authorAwori, Juliet O.
dc.contributor.authorBaillie, Vicky L.
dc.contributor.authorChipeta, James
dc.contributor.authorDeluca, Andrea N.
dc.contributor.authorDione, Michel
dc.contributor.authorDriscoll, Amanda J.
dc.contributor.authorHigdon, Melissa M.
dc.contributor.authorJatapai, Anchalee
dc.contributor.authorKarron, Ruth A.
dc.contributor.authorMazumder, Razib
dc.contributor.authorMoore, David P.
dc.contributor.authorMwansa, James
dc.contributor.authorNyongesa, Sammy
dc.contributor.authorProsperi, Christine
dc.contributor.authorSeidenberg, Phil
dc.contributor.authorSiludjai, Duangkamon
dc.contributor.authorSow, Samba O.
dc.contributor.authorTamboura, Boubou
dc.contributor.authorZeger, Scott L.
dc.contributor.authorMurdoch, David R.
dc.contributor.authorMadhi, Shabir A.
dc.date.accessioned2022-02-05T20:55:14Z
dc.date.available2022-02-05T20:55:14Z
dc.date.issued2017
dc.description.abstractDetection of pneumococcus by lytA polymerase chain reaction (PCR) in blood had poor diagnostic accuracy for diagnosing pneumococcal pneumonia in children in 9 African and Asian sites. We assessed the value of blood lytA quantification in diagnosing pneumococcal pneumonia. Methods. The Pneumonia Etiology Research for Child Health (PERCH) case-control study tested whole blood by PCR for pneumococcus in children aged 1–59 months hospitalized with signs of pneumonia and in age–frequency matched community controls. The distribution of load among PCR-positive participants was compared between microbiologically confirmed pneumococcal pneumonia (MCPP) cases, cases confirmed for nonpneumococcal pathogens, nonconfirmed cases, and controls. Receiver operating characteristic analyses determined the “optimal threshold” that distinguished MCPP cases from controls. Results. Load was available for 290 of 291 cases with pneumococcal PCR detected in blood and 273 of 273 controls. Load was higher in MCPP cases than controls (median, 4.0 × 103 vs 0.19 × 103 copies/mL), but overlapped substantially (range, 0.16–989.9 × 103 copies/mL and 0.01–551.9 × 103 copies/mL, respectively). The proportion with high load (≥2.2 log10 copies/mL) was 62.5% among MCPP cases, 4.3% among nonconfirmed cases, 9.3% among cases confirmed for a nonpneumococcal pathogen, and 3.1% among controls. Pneumococcal load in blood was not associated with respiratory tract illness in controls (P = .32). High blood pneumococcal load was associated with alveolar consolidation on chest radiograph in nonconfirmed cases, and with high (>6.9 log10 copies/mL) nasopharyngeal/oropharyngeal load and C-reactive protein ≥40 mg/L (both P < .01) in nonconfirmed cases but not controls. Conclusions. Quantitative pneumococcal PCR in blood has limited diagnostic utility for identifying pneumococcal pneumonia in individual children, but may be informative in epidemiological studies.en_US
dc.identifier.citationDeloria Knoll, M., Morpeth, S. C., Scott, J. A. G., Watson, N. L., Park, D. E., Baggett, H. C., ... & Mitchell, J. (2017). Evaluation of pneumococcal load in blood by polymerase chain reaction for the diagnosis of pneumococcal pneumonia in young children in the PERCH study. Clinical Infectious Diseases, 64(suppl_3), S357-S367. DOI: 10.1093/cid/cix149en_US
dc.identifier.other10.1093/cid/cix149
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1956
dc.language.isoenen_US
dc.publisherClinical Infectious Diseasesen_US
dc.subjectPneumoniaen_US
dc.subjectPneumococcusen_US
dc.subjectPCRen_US
dc.subjectBlooden_US
dc.subjectDiagnosisen_US
dc.titleEvaluation of Pneumococcal Load in Blood by Polymerase Chain Reaction for the Diagnosis of Pneumococcal Pneumonia in Young Children in the PERCH Studyen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Evaluation of Pneumococcal Load in Blood by Polymerase.pdf
Size:
782.25 KB
Format:
Adobe Portable Document Format
Description:
Article
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: