Advancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies

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dc.contributor.authorPaula, L. Marcet
dc.contributor.authorBrandon, Short
dc.contributor.authorAshley, Deas
dc.contributor.authorHong, Sun
dc.contributor.authorChelsea, Harrington
dc.contributor.authorShahzad, Shaukat
dc.contributor.authorMuhammad, Masroor Alam
dc.contributor.authorBaba, Marycelin
dc.contributor.authorAdedayo, Faneye
dc.contributor.authorNamuwulya, Prossy
dc.contributor.authorApostol, Lea Necitas
dc.contributor.authorElshaarawy, Tamer
dc.contributor.authorOdoom, John Kofi
dc.contributor.authorBorusPeter
dc.contributor.authorShelina Moonsamy
dc.contributor.authorYogolelo, Riziki
dc.contributor.authorZanga, Marie Claire Endegue
dc.contributor.authorTefera, Mesfin
dc.contributor.authorAnfumbom, K. W. Kfutwah
dc.contributor.authorSalmaan, Sharif
dc.contributor.authorVarja, Grabovac
dc.contributor.authorCara, C. Burns
dc.contributor.authorNancy, Gerlof
dc.date.accessioned2025-03-09T16:05:02Z
dc.date.available2025-03-09T16:05:02Z
dc.date.issued2025
dc.description.abstractIn the Global Polio Laboratory Network (GPLN), poliovirus (PV) screening results from acute flaccid paralysis (AFP) surveillance is based on virus isolation (VI) through cell culture, entailing long turnaround times and the amplification of live poliovirus. An alternative Direct Detection strategy (DD-ITD) for screening viral nucleic acid from stools, bypassing the need for virus culture, has been developed and extensively validated by GPLN partners. A multi-laboratory demonstration project was conceived to field-test the DD-ITD method by GPLN laboratories from the WHO African, Western Pacific, and Eastern Mediterranean regions, where wild serotype 1 or vaccine-derived polioviruses still circulate. Strategically selected laboratories were tasked to simultaneously process stool suspensions with the current gold-standard VI method and the new DD-ITD strategy. Results from 12 laboratories were compiled and analyzed to assess the quality of each RNA extraction and rRT-PCR run. Matched results for both methods of over 10,500 specimens showed an overall method agreement of 91%. All laboratories detected more PV presumptive positive samples with the DD-ITD strategy than with VI, but a large proportion of DD-ITD positive results (72%) were inconclusive or non-typeable, requiring confirmation through sequencing. A total of 298 (2.8%) samples were PV positive using both methods, 828 (7.9%) positive only for DD-ITD, and 62 (0.6%) positive only with VI. The DD-ITD overall performance, quality of results, and agreement between method results varied significantly across participating laboratories. DD-ITD implementation would entail building proficiency in advanced molecular laboratory techniques and data analysis, and increased demand for confirmatory sequencing.
dc.identifier.citationMarcet, P. L., Short, B., Deas, A., Sun, H., Harrington, C., Shaukat, S., ... & Gerloff, N. (2025). Advancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies. Microbiology Spectrum, 13(2), e02279-24. https://doi.org/10.1128/spectrum.02279-24
dc.identifier.urihttps://doi.org/10.1128/spectrum.02279-24
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/10087
dc.language.isoen
dc.publisherMicrobiology Spectrum
dc.titleAdvancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies
dc.typeArticle
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