A comparison of oral bacteriome isolated from periodontal pockets of participants with or without diabetes mellitus in Uganda: a case control study
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Date
2024-05
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central Ltd
Abstract
Abstract
Objective Diabetes mellitus predisposes patients to increased incidence and severe forms of periodontal disease.
Currently, information on the bacterial diversity of patients with diabetes mellitus and periodontitis in Uganda is
scanty. This study set out to describe the bacteria associated with periodontitis in patients with diabetes mellitus in
Uganda, as part of a larger study describing the association between periodontal disease and diabetes mellitus.
Results This was a case control involving 45 samples of gingival crevicular fluid collected from participants with
periodontitis, the cases being 26 participants with diabetes mellitus and controls 19 participants without diabetes
mellitus. Sequencing using the 16s Oxford nanopore long read protocol was followed by a bioinformatics analysis
pipeline for alpha and beta diversity indices in the two groups. Multivariate tests were done to determine the
differences in the bacterial composition in the two groups. Of the 739 Operational Taxonomic Units and 500 phyla
identified, 37.9% (280/739) were from participants with diabetes mellitus. Analysis of beta diversity revealed a
dissimilarity between the two study groups (CAP score=0) with a significant association noted between periodontitis
and the subgingival bacteria (P=0.001). Diabetes mellitus reduced the quantity and altered the composition of the
subgingival microbiome in the study participants.
Keywords Diabetes mellitus, Microbiome, OTUs, Periodontal pockets and periodontitis
Description
Keywords
Diabetes mellitus, Microbiome, OTUs, Periodontal pockets and periodontitis
Citation
Kiryowa, Haruna Muhmood, William Buwembo, Ian Guyton Munabi, et al. 'A Comparison of Oral Bacteriome Isolated from Periodontal Pockets of Participants with Or without Diabetes Mellitus in Uganda: A Case Control Study', BMC Research Notes, vol. 17/no. 1, (2024), pp. 146-146.