Evaluation of a New Temporary Immersion Bioreactor System for Micropropagation of Cultivars of Eucalyptus, Birch and Fir
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Forests
Abstract
The use of liquid instead of solid culture medium for the micropropagation of plants
offers advantages such as better access to medium components and scalability through possible
automation of the processes. The objective of this work was to compare a new temporary immersion
bioreactor (TIB) to solid medium culture for the micropropagation of a selection of tree species
micropropagated for commercial use: Nordmann fir (Abies nordmanniana (Steven) Spach), Eucalyptus
(E. grandis x E. urophylla), Downy birch (Betula pubescens Ehrh), and Curly birch (Betula pendula
var. carelica). Cultivation of explants in the TIB resulted in a significant increase of multiplication rate
and fresh weight of Eucalyptus and B. pendula, but not Betula pubescens. In addition, the fresh weight
of embryogenic tissue and the maturation frequency of somatic embryos increased significantly when
an embryogenic cell line of A. nordmanniana was cultivated in the TIB compared to solid culture
medium. These results demonstrate the potential for scaling up and automating micropropagation
by shoot multiplication and somatic embryogenesis in commercial tree species using a temporary
immersion bioreactor.
Description
Keywords
Forest trees, Temporary immersion, Somatic embryogenesis, Micropropagation
Citation
Businge, E., Trifonova, A., Schneider, C., Rödel, P., & Egertsdotter, U. (2017). Evaluation of a new temporary immersion bioreactor system for micropropagation of cultivars of eucalyptus, birch and fir. Forests, 8(6), 196. doi:10.3390/f8060196