Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis

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dc.contributor.authorYassine, Edriss
dc.contributor.authorGaliwango, Ronald
dc.contributor.authorSsengooba, Willy
dc.contributor.authorAshaba, Fred
dc.contributor.authorJoloba, Moses L.
dc.contributor.authorZalwango, Sarah
dc.contributor.authorWhalen, Christopher C.
dc.contributor.authorQuinn, Frederick
dc.date.accessioned2022-06-07T05:50:01Z
dc.date.available2022-06-07T05:50:01Z
dc.date.issued2021
dc.description.abstractTuberculosis (TB) is the leading cause of death in humans by a single infectious agent worldwide with approximately two billion humans latently infected with the bacterium Mycobacterium tuberculosis. Currently, the accepted method for controlling the disease is Tuberculosis Directly Observed Treatment Shortcourse (TB-DOTS). This program is not preventative and individuals may transmit disease before diagnosis, thus better understanding of disease transmission is essential. Using whole-genome sequencing and single nucleotide polymorphism analysis, we analyzed genomes of 145 M. tuberculosis clinical isolates from active TB cases from the Rubaga Division of Kampala, Uganda. We established that these isolates grouped into M. tuberculosis complex (MTBC) lineages 1, 2, 3, and 4, with the most isolates grouping into lineage 4. Possible transmission pairs containing ≤12 SNPs were identified in lineages 1, 3, and 4 with the prevailing transmission in lineages 3 and 4. Furthermore, investigating DNA codon changes as a result of specific SNPs in prominent virulence genes including plcA and plcB could indicate potentially important modifications in protein function. Incorporating this analysis with corresponding epidemiological data may provide a blueprint for the integration of public health interventions to decrease TB transmission in a region.en_US
dc.identifier.citationYassine, E., Galiwango, R., Ssengooba, W., Ashaba, F., Joloba, M. L., Zalwango, S., ... & Quinn, F. (2021). Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis. MicrobiologyOpen, 10(3), e1211. DOI: 10.1002/mbo3.1211en_US
dc.identifier.other10.1002/mbo3.1211
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3794
dc.language.isoenen_US
dc.publisherMicrobiologyOpenen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectSingle nucleotide polymorphismen_US
dc.subjectSocial networken_US
dc.subjectTransmissionen_US
dc.subjectTuberculosisen_US
dc.titleAssessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysisen_US
dc.typeArticleen_US
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