Acceptability of study procedures (self‑collected introital swabs, blood draws and stool sample collection) by students 10–16 years for an HPV vaccine effectiveness study: a pilot study
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Date
2016-03-16
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BMC Research Notes
Abstract
A cohort study was planned to evaluate vaccine immunogenicity and effect of malaria and helminth
co-infections on the bivalent Human papilloma virus (HPV) vaccine. The study would involve self collected introital
swabs, blood draws and stool sample collection. We therefore conducted a pilot study to assess the acceptability of
these procedures among the students and their parents.
A cross-sectional study among forty four students from two purposively selected primary schools of Western
Uganda. Exit interviews and two focus group discussions (FGD) (for parents) were conducted. Acceptability was
measured by willingness to undergo the procedures again, recommending the procedures to others as well as proportion
of introital swabs positive for β globulin. FGD determined acceptability of the parents and explored opinions
and perceptions that would influence their decisions. HPV-16/18 and β globulin deoxyribonucleic acid (DNA) were
analysed using a polymerase chain reaction (PCR) kit. All the students (100 %) in the study were willing to provide a
self- collected introital swab and a stool sample as well as recommending their friends while (86.3 %) were willing for
blood draws. There were 40/44 (90.1 %) self collected introital swabs that had positive result for human β globulin
though none of them was positive for HPV-16/18. In the FGD, it emerged that parents concerns were on the blood
draws and introital swab collection which were addressed. The study procedures were highly acceptable among this study population of students and their
parents. Follow-up to assess HPV vaccine effectiveness and factors that may influence the vaccine in this age group is
feasible.
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Citation
Nakalembe, M., Mutyaba, T., & Mirembe, F. (2016). Acceptability of study procedures (self-collected introital swabs, blood draws and stool sample collection) by students 10–16 years for an HPV vaccine effectiveness study: a pilot study. BMC Research Notes, 9(1), 170.