Bacterial community changes during composting of municipal crop waste using low technology methods as revealed by 16S rRNA1* and Department

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dc.contributor.authorTumuhairwe, John Baptist
dc.contributor.authorTenywa, John Stephen
dc.date.accessioned2021-12-02T20:06:32Z
dc.date.available2021-12-02T20:06:32Z
dc.date.issued2018
dc.description.abstractBacterial communities are actively involved in composting process but the environment within the compost influences their diversity, abundance and succession. In this study, the authors investigated the bacterial communities in tropical crop waste compost using pyrosequencing. Municipal crop wastes from the tropics (Uganda) were composted under four different low-technology methods. Samples were collected from the early thermophilic, late thermophilic, and mesophilic phases, and from mature compost. Pyrosequencing of the amplified variable V4 region of the 16s rDNA generated over 110 000 sequences. Chao1 and cluster analysis at 3% dissimilarity showed that bacterial community richness declined during the composting process. The community was dominated by a few bacterial taxa during the thermophilic phases. Species evenness increased as compost progressed to maturity despite a decline in the number of taxa over the successional progression. Bacterial community diversity, abundance and succession changed with the composting method. This pattern of diversity may be attributed to competition and selection during the microbial succession. A total of 22 phyla and 513 genera were identified from all the methods in the entire composting process. The most abundant phyla were Proteobacteria, Firmicutes, Bacteriodes and Actinobacteria. Pyrosequencing provided more information on compost bacterial community diversity and abundance than previously used molecular methods. Several novel bacteria existing in tropical crop waste compost remained unclassified.en_US
dc.identifier.citationTumuhairwe, J. B., & Tenywa, J. S. (2018). Bacterial community changes during composting of municipal crop waste using low technology methods as revealed by 16S rRNA. African Journal of Environmental Science and Technology, 12(6), 209-221. https://doi.org/10.5897/AJEST2018.2479en_US
dc.identifier.issn1996-0786
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/168
dc.language.isoenen_US
dc.publisherAfrican Journal of Environmental Science and Technologyen_US
dc.subject16S rRNA, succession, compost, bacterial community, pyrosequencing.en_US
dc.titleBacterial community changes during composting of municipal crop waste using low technology methods as revealed by 16S rRNA1* and Departmenten_US
dc.typeArticleen_US
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