Identification of Echinococcus granulosus strains using polymerase chain reaction–restriction fragment length polymorphism amongst livestock in Moroto district, Uganda

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dc.contributor.authorChamai, Martin
dc.contributor.authorOmadang, Leonard
dc.contributor.authorErume, Jospeh
dc.contributor.authorOcaido, Michael
dc.contributor.authorOba, Peter
dc.contributor.authorOthieno, Emmanuel
dc.contributor.authorBonaventure, Straton
dc.contributor.authorKitibwa, Annah
dc.date.accessioned2025-03-11T18:05:18Z
dc.date.available2025-03-11T18:05:18Z
dc.date.issued2016
dc.description.abstractA descriptive study was conducted to identify the different strains of Echinococcus granulosus occurring in livestock in Moroto district, Uganda. Echinococcus cysts from 104 domestic animals, including cattle, sheep, goats and camels, were taken and examined by microscopy, polymerase chain reaction with restriction fragment length polymorphism and Sanger DNA sequencing. Echinococcus granulosus genotypes or strains were identified through use of Bioinformatics tools: BioEdit, BLAST and MEGA6. The major finding of this study was the existence of a limited number of E. granulosus genotypes from cattle, goats, sheep and camels. The most predominant genotype was G1 (96.05%), corresponding to the common sheep strain. To a limited extent (3.95%), the study revealed the existence of Echinococcus canadensis G6/7 in three (n = 3) of the E. granulosus-positive samples. No other strains of E. granulosus were identified. It was concluded that the common sheep strain of Echinococcus sensu stricto and G6/7 of E. canadensis were responsible for echinococcal disease in Moroto district, Uganda.
dc.identifier.citationChamai, M., Omadang, L., Erume, J., Ocaido, M., Oba, P., Othieno, E. et al., 2016, ‘Identification of Echinococcus granulosus strains using polymerase chain reaction–restriction fragment length polymorphism amongst livestock in Moroto district, Uganda’, Onderstepoort Journal of Veterinary Research 83(1), a1068. http://dx.doi.org/10.4102/ ojvr.v83i1.1068
dc.identifier.issn2219-0635
dc.identifier.issn0030-2465
dc.identifier.urihttp://dx.doi.org/10.4102/ ojvr.v83i1.1068
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/10116
dc.language.isoen
dc.publisherOnderstepoort Journal of Veterinary Research
dc.titleIdentification of Echinococcus granulosus strains using polymerase chain reaction–restriction fragment length polymorphism amongst livestock in Moroto district, Uganda
dc.typeArticle
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