Genetic Relationships of Cassava Genotypes That are Susceptible or Tolerant to Cassava Brown Streak Disease in Uganda
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Date
2013
Authors
Abaca, Alex
Kawuki, Robert
Tukamuhabwa, Phenihas
Baguma, Yona
Pariyo, Anthony
Alicai, Titus
Omongo, C. Christopher
Abidrabo, Phillips
Katono, Kasifa
Bua, Anton
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Agricultural Science
Abstract
A total of 99 cassava genotypes whose field reaction to cassava brown streak disease (CBSD) was known, were
assayed with 30 simple sequence repeat (SSR) markers to establish their genetic parallels. Two categories of
CBSD reaction were considered: CBSD-susceptible genotypes (characterized by > 60% root and foliar CBSD
incidence), and CBSD-tolerant genotypes (characterized by < 15% root and foliar CBSD incidence). DNA was
extracted from leaf samples using the miniprep method and genotyped using ABI 3730 DNA sequencer. The test
genotypes at 0.02 similarity coefficient, CBSD-tolerant and CBSD-susceptible genotypes clustered into 5 main
sub-clusters. When data were subjected to principle component analysis (PCA) irrespective of the CBSD reaction
grade, the first three principal components accounted for 68% of the total genetic variation. Despite having
different number of individuals, the observed heterozygosity (Ho) for CBSD-susceptible (Ho = 0.58) and
CBSD-tolerant (Ho = 0.66) were comparable and provide scope for long-term CBSD breeding and/or gene
tapping.
Description
Keywords
Cassava brown streak virus, Cluster analysis, Foliar symptoms, Simple sequence repeat marker
Citation
Abaca, A., Kawuki, R., Tukamuhabwa, P., Baguma, Y., Pariyo, A., Alicai, T., ... & Bua, A. (2013). Genetic relationships of cassava genotypes that are susceptible or tolerant to cassava brown streak disease in Uganda. Journal of Agricultural Science, 5(7), 107. http://dx.doi.org/10.5539/jas.v5n7p107