Small-RNA Deep Sequencing Reveals Arctium tomentosum as a Natural Host of Alstroemeria virus X and a New Putative Emaravirus

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dc.contributor.authorBi, Yaqi
dc.contributor.authorTugume, Arthur K.
dc.contributor.authorValkonen, Jari P. T.
dc.date.accessioned2022-03-07T13:12:46Z
dc.date.available2022-03-07T13:12:46Z
dc.date.issued2012
dc.description.abstractArctium species (Asteraceae) are distributed worldwide and are used as food and rich sources of secondary metabolites for the pharmaceutical industry, e.g., against avian influenza virus. RNA silencing is an antiviral defense mechanism that detects and destroys virus-derived double-stranded RNA, resulting in accumulation of virus-derived small RNAs (21–24 nucleotides) that can be used for generic detection of viruses by small-RNA deep sequencing (SRDS). Methodology/Principal Findings: SRDS was used to detect viruses in the biennial wild plant species Arctium tomentosum (woolly burdock; family Asteraceae) displaying virus-like symptoms of vein yellowing and leaf mosaic in southern Finland. Assembly of the small-RNA reads resulted in contigs homologous to Alstroemeria virus X (AlsVX), a positive/single-stranded RNA virus of genus Potexvirus (family Alphaflexiviridae), or related to negative/single-stranded RNA viruses of the genus Emaravirus. The coat protein gene of AlsVX was 81% and 89% identical to the two AlsVX isolates from Japan and Norway, respectively. The deduced, partial nucleocapsid protein amino acid sequence of the emara-like virus was only 78% or less identical to reported emaraviruses and showed no variability among the virus isolates characterized. This virus—tentatively named as Woolly burdock yellow vein virus—was exclusively associated with yellow vein and leaf mosaic symptoms in woolly burdock, whereas AlsVX was detected in only one of the 52 plants tested. Conclusions/Significance: These results provide novel information about natural virus infections in Acrtium species and reveal woolly burdock as the first natural host of AlsVX besides Alstroemeria (family Alstroemeriaceae). Results also revealed a new virus related to the recently emerged Emaravirus genus and demonstrated applicability of SRDS to detect negativestrand RNA viruses. SRDS potentiates virus surveys of wild plants, a research area underrepresented in plant virology, and helps reveal natural reservoirs of viruses that cause yield losses in cultivated plants.en_US
dc.identifier.citationBi Y, Tugume AK, Valkonen JPT (2012) Small-RNA Deep Sequencing Reveals Arctium tomentosum as a Natural Host of Alstroemeria virus X and a New Putative Emaravirus. PLoS ONE 7(8): e42758. doi:10.1371/journal.pone.0042758en_US
dc.identifier.other10.1371/journal.pone.0042758
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2490
dc.language.isoenen_US
dc.publisherPLoS ONEen_US
dc.subjectSmall-RNA Deep Sequencingen_US
dc.subjectArctium tomentosumen_US
dc.subjectNatural Hosten_US
dc.subjectAlstroemeria virus Xen_US
dc.subjectNew Putative Emaravirusen_US
dc.titleSmall-RNA Deep Sequencing Reveals Arctium tomentosum as a Natural Host of Alstroemeria virus X and a New Putative Emaravirusen_US
dc.typeArticleen_US
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