Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virus isolates

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dc.contributor.authorDawit B, Kidanemariam
dc.contributor.authorAmit C, Sukal
dc.contributor.authorAdane D, Abraham
dc.contributor.authorJoyce N, Njuguna
dc.contributor.authorFrancesca, Stomeo
dc.contributor.authorJames L, Dale
dc.contributor.authorAnthony P, James, Robert M. Harding
dc.contributor.authorAnthony P, James
dc.contributor.authorRobert M, Harding
dc.date.accessioned2023-02-06T11:10:46Z
dc.date.available2023-02-06T11:10:46Z
dc.date.issued2021-07-30
dc.description.abstractAbstract Taro (Colocasia esculenta) and tannia (Xanthosoma sp.) plants growing in 25 districts across Ethiopia, Kenya, Tanzania and Uganda were surveyed for four RNA viruses. Leaf samples from 392 plants were tested for cucumber mosaic virus (CMV), dasheen mosaic virus (DsMV), taro vein chlorosis virus (TaVCV) and Colocasia bobone disease-associated virus (CBDaV) by RT-PCR. No samples tested positive for TaVCV or CBDaV, while CMV was only detected in three tannia samples with mosaic symptoms from Uganda. DsMV was detected in 40 samples, including 36 out of 171 from Ethiopia, one out of 94 from Uganda and three out of 41 from Tanzania, while none of the 86 samples from Kenya tested positive for any of the four viruses. The complete genomes of nine DsMV isolates from East Africa were cloned and sequenced. Phylogenetic analyses based on the amino acid sequence of the DsMV CP-coding region revealed two distinct clades. Isolates from Ethiopia were distributed in both clades, while samples from Uganda and Tanzania belong to different clades. Seven possible recombination events were identified from the analysis carried out on the available 15 full-length DsMV isolates. Nucleotide substitution ratio analysis revealed that all the DsMV genes are under strong negative selection pressure.en_US
dc.description.sponsorshipAfrican Biosciences Challenge Fund; Swedish International Development Agency; UK Department for International Development; Bill and Melinda Gates Foundation; Syngenta Foundation for Sustainable Agriculture; Australian Department of Foreign Affairs and Tradeen_US
dc.identifier.citationKidanemariam, D. B., Sukal, A. C., Abraham, A. D., Njuguna, J. N., Stomeo, F., Dale, J. L., James, A. P., & Harding, R. M. (2022). Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virus isolates. Annals of Applied Biology, 180( 2), 211– 223. https://doi.org/10.1111/aab.12725en_US
dc.identifier.issn0003-4746
dc.identifier.issn1744-7348 (Online)
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7586
dc.language.isoenen_US
dc.publisherJohn Wiley & Sons, Ltden_US
dc.subjectaroids, cucumber mosaic virus, Ethiopia, Kenya, plant viruses, potyvirus, rhabdovirus, Tanzania, Ugandaen_US
dc.titleIncidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virus isolatesen_US
dc.typeArticleen_US
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