Genetic diversity and geographic distribution of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) genotypes associated with cassava in East Africa
Loading...
Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Ecology and Evolution
Abstract
The genetic variability of whitefly (Bemisia tabaci) species, the vectors of cassava
mosaic begomoviruses (CMBs) in cassava growing areas of Kenya, Tanzania,
and Uganda, was investigated through comparison of partial sequences of the
mitochondria cytochrome oxidase I (mtCOI) DNA in 2010/11. Two distinct
species were obtained including sub-Saharan Africa 1 (SSA1), comprising of
two sub-clades (I and II), and a South West Indian Ocean Islands (SWIO) species.
Among the SSA1, sub-clade I sequences shared a similarity of 97.8–99.7%
with the published Uganda 1 genotypes, and diverged by 0.3–2.2%. A pairwise
comparison of SSA1 sub-clade II sequences revealed a similarity of 97.2–99.5%
with reference southern Africa genotypes, and diverged by 0.5–2.8%. The SSA1
sub-clade I whiteflies were widely distributed in East Africa (EA). In comparison,
the SSA1 sub-clade II whiteflies were detected for the first time in the EA
region, and occurred predominantly in the coast regions of Kenya, southern
and coast Tanzania. They occurred in low abundance in the Lake Victoria Basin
of Tanzania and were widespread in all four regions in Uganda. The SWIO species
had a sequence similarity of 97.2–97.7% with the published Reunion
sequence and diverged by 2.3–2.8%. The SWIO whiteflies occurred in coast
Kenya only. The sub-Saharan Africa 2 whitefly species (Ug2) that was associated
with the severe CMD pandemic in Uganda was not detected in our study
Description
Keywords
Cytochrome oxidase I (mtCOI), Genetic diversity, Geographic distribution, Mitochondria, Whitefly
Citation
Mugerwa, H., Rey, M. E., Alicai, T., Ateka, E., Atuncha, H., Ndunguru, J., & Sseruwagi, P. (2012). Genetic diversity and geographic distribution of B emisia tabaci (G ennadius)(H emiptera: A leyrodidae) genotypes associated with cassava in East Africa. Ecology and Evolution, 2(11), 2749-2762. doi: 10.1002/ece3.379