Short Communication Colony-Forming Hematopoietic Progenitor Cells Are Not Preferentially Infected by HIV Type 1 Subtypes A and D in Vivo
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
AIDS research and human retroviruses
Abstract
HIV subtype C has previously been shown to infect hematopoietic progenitor cells (HPCs) at a significantly
higher rate than subtype B. To better understand the subtype-specific nature of HPC infection, we examined
the prevalence of HPC infection in vivo by HIV-1 subtypes A and D. HIV-1 infection of HPC was examined in
40 individuals, 19 infected with subtype A and 21 with subtype D, using a single colony assay format. DNA
from 1177 extracted colonies was tested for integrated viral DNA of the p24 gene. Four colonies were found to
be stably infected, three of 462 colonies (0.65%) from HIV-1A-infected individuals (1/19 individuals) and one
of 715 colonies (0.14%) from HIV-1D-infected individuals (1/22 individuals). These rates of colony infection
were comparable to the rates observed in PBMCs from the same subjects. Additionally, no correlation was
observed between cell colony density and circulating viral load or proviral load. Our findings suggest that
HIV-1 subtypes A and D do not preferentially infect colony-forming HPCs over mature HIV target cells
in vivo.
Description
Keywords
Short Communication Colony-Forming Hematopoietic Progenitor Cells, Infected, HIV Type 1 Subtypes A and D in Vivo
Citation
Mullis, C. E., Oliver, A. E., Eller, L. A., Guwatudde, D., Mueller, A. C., Eller, M. A., ... & Redd, A. D. (2012). Colony-forming hematopoietic progenitor cells are not preferentially infected by HIV type 1 subtypes A and D in vivo. AIDS research and human retroviruses, 28(9), 1119-1123. DOI: 10.1089/aid.2011.0179