In vitro Antibacterial Activity of Ocimum suave Essential Oils against Uropathogens Isolated from Patients in Selected Hospitals in Bushenyi District, Uganda
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
British Microbiology Research Journal
Abstract
Aims—To determine antibacterial activity of Ocimum suave essential oils against bacterial uropathogens.
Study Design—A cross sectional and experimental study.
Place and Duration of Study—Six selected hospitals in Bushenyi District, Uganda between June 2012 and July 2013.
Methodology—Clean catch midstream urine samples were collected and inoculated on Cystine Lysine Electrolyte Deficient (CLED) agar. The plates were incubated at 37°C for 24hrs to 48hrs. The O. suave essential oils were extracted by hydrodistillation of leaves for 4hrs using a Clevenger apparatus. The oil was collected and dried over anhydrous sodium sulphate (Na2SO4) and kept at 4°C till further use. The antimicrobial activity of O. suave essential oils against isolates was determined by agar well method. The MIC of O. suave essential oil extract was carried out by microbroth dilution method.
Results—Of the three hundred (300) midstream urine samples collected, 67(22.33%) had significant bacterial growth. Escherichia coli is the most common isolate (61.19%, n = 41).
Description
Keywords
Aromatic medicinal plants, bacteriauria, E. coli, resistance
Citation
Tibyangye, J., Okech, M. A., Nyabayo, J. M., & Nakavuma, J. L. (2015). In vitro antibacterial activity of Ocimum suave essential oils against uropathogens isolated from patients in selected hospitals in Bushenyi district, Uganda. British Microbiology Research Journal, 8(3), 489. doi:10.9734/BMRJ/2015/17526.