Identification of candidate genes associated with resistance to bruchid (Callosobruchus maculatus) in cowpea
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Date
2019
Authors
Miesho, Belay
Hailay, Mehari
Msiska, Ulemu
Bruno, Awio
Malinga, Geoffrey M.
Obia Ongom, Patrick
Edema, Richard
Gibson, Paul
Rubaihayo, Patrick
Kyamanywa, Samuel
Journal Title
Journal ISSN
Volume Title
Publisher
Plant Breeding
Abstract
Cowpea is an important legume crop widely grown in sub‐Saharan Africa for food and
feed. However, it is largely challenged by bruchid, a serious storage pest resulting in
losses in quantity and quality of grains. Therefore, this research was designed to contribute
to the breeding of cowpea resistance to bruchid through the identification of
candidate genes associated with resistance to bruchid. A total of 217 mini‐core cowpea
accessions were genotyped and phenotyped for their reactions to bruchid. To determine
the genomic regions linked with bruchid resistance, 41,948 polymorphic SNP markers
were used. Genome‐wide association study identified 11 SNPs linked to the average
number of eggs, holes, insect emergence and development period and Dobie susceptibility
index. Gene search via Phytozome identified six candidate genes (Vigun08g132300,
Vigun08g158000, Vigun06g053700, Vigun02g131000, Vigun01g234900 and
Vigun01g201900) associated with the resistance traits. These candidate genes could be
incorporated into the farmers preferred but susceptible cowpea varieties to bruchid. The
SNP markers associated with the resistance traits can be used in marker‐assisted breeding
for accurate and rapid screening of cowpea resistant genotypes to bruchid.
Description
Keywords
Genome‐wide association, Genomic region, SNP, Vigun
Citation
Miesho, B., Hailay, M., Msiska, U., Bruno, A., Malinga, G. M., Obia Ongom, P., ... & Kyamanywa, S. (2019). Identification of candidate genes associated with resistance to bruchid (Callosobruchus maculatus) in cowpea. Plant Breeding, 138(5), 605-613. DOI: 10.1111/pbr.12705