Phenotypic and genotypic screening for rust resistance in common bean germplasm in Uganda

Simple item page
dc.contributor.authorOdogwu, Blessing A.
dc.contributor.authorNkalubo, Stanley T.
dc.contributor.authorMukankusi, Clare
dc.contributor.authorOdong, Thomas
dc.contributor.authorAwale, Halima E.
dc.contributor.authorPatrick, Rubaihayo
dc.contributor.authorKelly, James D.
dc.date.accessioned2022-02-02T09:40:49Z
dc.date.available2022-02-02T09:40:49Z
dc.date.issued2017
dc.description.abstractRust caused by Uromyces appendiculatus (Pers., Pers.) Unger is one of the major foliar diseases of common bean (Phaseolus vulgaris) in Uganda. The use of host resistance remains the best option in managing this disease. The objective of this study was to identify sources of broad-spectrum rust resistance in common bean germplasm including landraces, commercial and introduced genotypes using a combination of phenotypic and genotypic screening with simple sequence repeats (SSRs) markers located on chromosome Pv04. A total of 138 cultivars were field screened from 2014 and 2015 using alpha lattice design. The variance and correlation of disease incidence, area under the disease progression curve (AUDPC) and total grain yield were computed using GenStat. The polymorphism information content of the cultivars was determined, and the association of the markers and the disease resistance traits were analyzed using PowerMarker and TASSEL respectively. Resistance of each cultivar was compared to the presence and absence of amplified markers. There were highly significant differences (P < 0.001) among the cultivars for disease incidence, AUDPC and total grain yield and a strong correlation (P < 0.001) between disease incidence and AUDPC in both years. The SSR markers, BARC_PV_SSR04725, bean_ssr_0778 and bean_ssr_2892 were observed to be associated (P ≤0.05) with rust resistance. The two screening methods identified cultivars which included local cultivars, Nabufumbo, Kapchorwa white, and NABE as new sources of rust resistance. This study identified sources of rust resistance that would be useful in the bean breeding programmes in Uganda.en_US
dc.identifier.citationOdogwu, Blessing A.; Nkalubo, Stanley T.; Mukankusi, Clare; Odong, Thomas; Awale, Halima E.; Rubaihayo, Patrick; Kelly, James D.. 2017. Phenotypic and genotypic screening for rust resistance in common bean germplasm in Uganda. Euphytica. 213: 49. https://dx.doi.org/10.1007/s10681-016-1795-yen_US
dc.identifier.urihttps://dx.doi.org/10.1007/s10681-016-1795-y
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1740
dc.language.isoenen_US
dc.publisherEuphyticaen_US
dc.subjectUromyces appendiculatusen_US
dc.subjectPhaseolus vulgarisen_US
dc.subjectSSR markersen_US
dc.subjectBroad spectrum rust resistanceen_US
dc.subjectAUDPCen_US
dc.titlePhenotypic and genotypic screening for rust resistance in common bean germplasm in Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Phenotypic and genotypic screening for rust resistance in common bean germplasm in Uganda.pdf
Size:
785.34 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences