Phylogenetic diversity of Rhizobium species recovered from nodules of common beans (Phaseolus vulgaris L.) in fields in Uganda: R. phaseoli, R. etli, and R. hidalgonense
| dc.contributor.author | Aserse, Aregu Amsalu; | |
| dc.contributor.author | Nimusiima, Jean; | |
| dc.contributor.author | Tumuhairwe, John Baptist ; | |
| dc.contributor.author | Yli-Halla, Markku; | |
| dc.contributor.author | Lindström, Kristina | |
| dc.date.accessioned | 2025-04-03T12:35:52Z | |
| dc.date.available | 2025-04-03T12:35:52Z | |
| dc.date.issued | 2024-10 | |
| dc.description.abstract | A total of 75 bacterial isolates were obtained from nodules of beans cultivated across 10 sites in six agro-ecological zones in Uganda. Using recA gene sequence analysis, 66 isolates were identified as members of the genus Rhizobium, while 9 were related to Agrobacterium species. In the recA gene tree, most Rhizobium strains were classified into five recognized species. Phylogenetic analysis based on six concatenated sequences (recA-rpoB-dnaK-glnII-gyrB-atpD) placed 32 representative strains into five distinct Rhizobium species, consistent with the species groups observed in the recA gene tree: R. phaseoli, R. etli, R. hidalgonense, R. ecuadorense, and R. sophoriradicis, with the first three being the predominant. The rhizobial strains grouped into three nodC subclades within the symbiovar phaseoli clade, encompassing strains from distinct phylogenetic groups. This pattern reflects the conservation of symbiotic genes, likely acquired through horizontal gene transfer among diverse rhizobial species. The 32 representative strains formed symbiotic relationships with host beans, while the Agrobacterium strains did not form nodules and lacked symbiotic genes. Multivariate analysis revealed that species distribution was influenced by the environmental factors of the sampling sites, emphasizing the need to consider these factors in future effectiveness studies to identify effective nitrogen-fixing strains for specific locations.A total of 75 bacterial isolates were obtained from nodules of beans cultivated across 10 sites in six agro-ecological zones in Uganda. Using recA gene sequence analysis, 66 isolates were identified as members of the genus Rhizobium, while 9 were related to Agrobacterium species. In the recA gene tree, most Rhizobium strains were classified into five recognized species. Phylogenetic analysis based on six concatenated sequences (recA-rpoB-dnaK-glnII-gyrB-atpD) placed 32 representative strains into five distinct Rhizobium species, consistent with the species groups observed in the recA gene tree: R. phaseoli, R. etli, R. hidalgonense, R. ecuadorense, and R. sophoriradicis, with the first three being the predominant. The rhizobial strains grouped into three nodC subclades within the symbiovar phaseoli clade, encompassing strains from distinct phylogenetic groups. This pattern reflects the conservation of symbiotic genes, likely acquired through horizontal gene transfer among diverse rhizobial species. The 32 representative strains formed symbiotic relationships with host beans, while the Agrobacterium strains did not form nodules and lacked symbiotic genes. Multivariate analysis revealed that species distribution was influenced by the environmental factors of the sampling sites, emphasizing the need to consider these factors in future effectiveness studies to identify effective nitrogen-fixing strains for specific locations. MEDLINE - Academic | |
| dc.description.sponsorship | This work was supported by the EU–Africa research and innovation partnership on food and nutrition security and sustainable agriculture through the LEAP-AGRI project known as the PASUSI project, for which the Academy of Finland contributed funds (No. 319 874). Additionally, this work was supported by the Healthy Pulse project granted by the Finnish Cultural Foundation (No. 0116947-3) | |
| dc.identifier.citation | Aserse, Aregu Amsalu, Jean Nimusiima, John Baptist Tumuhairwe, et al. 'Phylogenetic Diversity of Rhizobium Species Recovered from Nodules of Common Beans (Phaseolus Vulgaris L.) in Fields in Uganda: R. Phaseoli, R. Etli, and R. Hidalgonense', FEMS Microbiology Ecology, vol. 100/no. 11, (2024), . | |
| dc.identifier.issn | ISSN 1574-6941, 0168-6496 | |
| dc.identifier.issn | EISSN 1574-6941 | |
| dc.identifier.uri | https://nru.uncst.go.ug/handle/123456789/10345 | |
| dc.language.iso | en | |
| dc.publisher | Oxford University Press | |
| dc.title | Phylogenetic diversity of Rhizobium species recovered from nodules of common beans (Phaseolus vulgaris L.) in fields in Uganda: R. phaseoli, R. etli, and R. hidalgonense | |
| dc.type | Article |