Bovine leptospirosis in abattoirs in Uganda: molecular detection and risk of exposure among workers

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dc.contributor.authorAlinaitwe, Lordrick
dc.contributor.authorKankya, Clovice
dc.contributor.authorAllan, Kathryn J.
dc.contributor.authorRodriguez-Campos, Sabrina
dc.contributor.authorTorgerson, Paul
dc.contributor.authorDreyfus, Anou
dc.date.accessioned2022-02-04T14:23:16Z
dc.date.available2022-02-04T14:23:16Z
dc.date.issued2019
dc.description.abstractLeptospirosis is a zoonotic bacterial disease reported worldwide. In Uganda, seropositivity has been reported in both humans and domesticated animals, including cattle. However, it remains unknown whether cattle are shedding leptospires and thus acting as potential source for human leptospirosis. We conducted this cross-sectional study in two cattle abattoirs in Kampala, Uganda between June and July 2017. Kidney and urine samples from 500 cattle sourced from across the country were analyzed by real-time PCR to establish the prevalence of Leptospira positive cattle and risk of exposure to abattoir workers. The species of infecting Leptospira was determined by amplification of secY gene and compared to reference sequences published in GenBank. Of 500 cattle tested, (7.2%) had Leptospira DNA in their kidneys (carriers), (5.8%) in their urine (shedders); with an overall prevalence (kidney and/or urine) of 8.8%. Leptospira borgpetersenii 2 was confirmed as the infecting species in three cattle and Leptospira kirschneri in one animal. Male vs female cattle (OR= 3, P–value 0.003), exotic vs local breeds (OR= 21.3, P–value 0.002) or cattle from Western Uganda (OR= 4.4, P–value 0.001) and from regions across the border 28 (OR= 3.3, P–value 0.032) vs from the central region were more likely to be Leptospira positive. The daily risk of exposure of abattoir workers to ≥1 (kidney and/or urine) positive carcass ranged from 27% (95% credibility interval 18.6 – 52.3) to 100% (95% CI 91.0 – 100.0), with halal butchers and pluck inspectors being at highest risk. In conclusion, cattle slaughtered at abattoirs in Uganda carry and shed pathogenic Leptospira species; and this may pose occupation-related risk of exposure among workers in these abattoirs, with workers who handle larger numbers of animals being at higher risk.en_US
dc.identifier.citationAlinaitwe, L., Kankya, C., Allan, K. J., Rodriguez-Campos, S., Torgerson, P. and Dreyfus, A. (2019) Bovine leptospirosis in abattoirs in Uganda: molecular detection and risk of exposure among workers. Zoonoses and Public Health, 66(6), pp. 636-646. http://dx.doi.org/10.1111/zph.12616en_US
dc.identifier.urihttp://dx.doi.org/10.1111/zph.12616
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1903
dc.language.isoenen_US
dc.publisherZoonoses and Public Healthen_US
dc.subjectAbattoiren_US
dc.subjectLeptospiraen_US
dc.subjectLeptospirosisen_US
dc.subjectReal-time PCRen_US
dc.subjectRisk of exposureen_US
dc.titleBovine leptospirosis in abattoirs in Uganda: molecular detection and risk of exposure among workersen_US
dc.typeArticleen_US
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