The Role of Molecular Testing in Pediatric Meningitis Surveillance in Southern and East African Countries, 2008–2017

Simple item page
dc.contributor.authorPlessis, Mignon du
dc.contributor.authorGouveia, Linda de
dc.contributor.authorFreitas, Cesar
dc.contributor.authorAsamene Abera, Negga
dc.contributor.authorMugisha, David
dc.contributor.authorNalumansi, Esther
dc.contributor.authorOdongkara, Moses
dc.contributor.authorLukwesa-Musyani, Chileshe
dc.contributor.authorLessa, Fernanda C.
dc.contributor.authorGottberg, Anne von
dc.date.accessioned2022-05-02T22:02:23Z
dc.date.available2022-05-02T22:02:23Z
dc.date.issued2021
dc.description.abstractAs part of the global Invasive Bacterial Vaccine-Preventable Diseases Surveillance Network, 12 African countries referred cerebrospinal fluid (CSF) samples to South Africa’s regional reference laboratory. We evaluated the utility of realtime polymerase chain reaction (PCR) in detecting and serotyping/grouping Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae (HNS). Methods. From 2008 to 2017, CSF samples collected from children <5 years old with suspected meningitis underwent routine microbiology testing in-country, and 11 680 samples were submitted for HNS PCR at the regional reference laboratory. Unconditional logistic regression, with adjustment for geographic location, was performed to identify factors associated with PCR positivity. Results. The overall HNS PCR positivity rate for all countries was 10% (1195 of 11 626 samples). In samples with both PCR and culture results, HNS PCR positivity was 11% (744 of 6747 samples), and HNS culture positivity was 3% (207 of 6747). Molecular serotype/serogroup was assigned in 75% of PCR-positive specimens (762 of 1016). Compared with PCR-negative CSF samples, PCRpositive samples were more often turbid (adjusted odds ratio, 6.80; 95% confidence interval, 5.67–8.17) and xanthochromic (1.72; 1.29–2.28), had elevated white blood cell counts (6.13; 4.71–7.99) and high protein concentrations (5.80; 4.34–7.75), and were more often HNS culture positive (32.70; 23.18–46.12). Conclusion. PCR increased detection of vaccine-preventable bacterial meningitis in countries where confirmation of suspected meningitis cases is impeded by limited culture capacity.en_US
dc.identifier.citationdu Plessis, M., de Gouveia, L., Freitas, C., Abera, N. A., Lula, B. S., Raboba, J. L., ... & von Gottberg, A. (2021). The Role of Molecular Testing in Pediatric Meningitis Surveillance in Southern and East African Countries, 2008–2017. The Journal of infectious diseases, 224(Supplement_3), S194-S203. DOI: 10.1093/infdis/jiab092en_US
dc.identifier.other10.1093/infdis/jiab092
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3169
dc.language.isoenen_US
dc.publisherThe Journal of infectious diseasesen_US
dc.subjectPediatric bacterial meningitis surveillanceen_US
dc.subjectAfricaen_US
dc.subjectReal-time PCRen_US
dc.subjectMolecular testingen_US
dc.subjectIB-VPD meningitis pathogensen_US
dc.titleThe Role of Molecular Testing in Pediatric Meningitis Surveillance in Southern and East African Countries, 2008–2017en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
The Role of Molecular Testing in Pediatric Meningitis.pdf
Size:
338.07 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Medical and Health Sciences