Evaluation of cytokine responses against novel Mtb antigens as diagnostic markers for TB disease
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Infection
Abstract
We investigated the accuracy of host markers detected in Mtb antigenstimulated
whole blood culture supernatant in the diagnosis of TB.
Methods: Prospectively, blood from 322 individuals with presumed TB disease from six African
sites was stimulated with four different Mtb antigens (Rv0081, Rv1284, ESAT-6/CFP-10, and
Rv2034) in a 24 h whole blood stimulation assay (WBA). The concentrations of 42 host markers
in the supernatants were measured using the Luminex multiplex platform. Diagnostic biosignatures
were investigated through the use of multivariate analysis techniques.
Results: 17% of the participants were HIV infected, 106 had active TB disease and in 216 TB was
excluded. Unstimulated concentrations of CRP, SAA, ferritin and IP-10 had better discriminating
ability than markers from stimulated samples. Accuracy of marker combinations by general
discriminant analysis (GDA) identified a six analyte model with 77% accuracy for TB cases
and 84% for non TB cases, with a better performance in HIV uninfected patients.
Conclusions: A biosignature of 6 cytokines obtained after stimulation with four Mtb antigens
has moderate potential as a diagnostic tool for pulmonary TB disease individuals and stimulated
marker expression had no added value to unstimulated marker performance.
Description
Keywords
Biosignature, Host markers, Antigen
Citation
Awoniyi, D. O., Teuchert, A., Sutherland, J. S., Mayanja-Kizza, H., Howe, R., Mihret, A., ... & Cliff, J. M. (2016). Evaluation of cytokine responses against novel Mtb antigens as diagnostic markers for TB disease. Journal of Infection, 73(3), 219-230. http://dx.doi.org/10.1016/j.jinf.2016.04.036