A Comparative Analysis of Conventional and Marker-Assisted Selection Methods in Breeding Maize Streak Virus Resistance in Maize
Loading...
Date
2009
Journal Title
Journal ISSN
Volume Title
Publisher
Crop Science
Abstract
Reliable information regarding comparative
advantage of marker-assisted selection (MAS)
over conventional selection (CS) in breeding for
maize streak virus (MSV) resistance in maize
(Zea mays L.) is scarcely available. A comparative
study was, therefore, conducted to determine
the effi ciency of both methods in breeding
for MSV resistance in Uganda. Backcross and
selfed-progenies were derived from inbred lines
CML202 (resistant), CML321, and CML384 (susceptible)
using MAS and CS. The experimental
lines and their testcross progenies were evaluated
for MSV resistance and yield across three
locations. Although both breeding approaches
were effective in generating MSV-resistant lines,
disease incidence was higher in populations
under CS (79%) than MAS (62%). A similar trend
was observed for area under disease progress
curve. However, an equal number of lines generated
by MAS and CS displayed high yield potential
and MVS resistance in testcrosses. Because
all required DNA analysis was performed in an
existing laboratory and on a well-characterized
quantitative trait locus, costs of capital, equipment
maintenance, and marker development
were excluded in costing the MAS procedure.
Considering total running costs, MAS was
cheaper than CS by 26%, which was realized by
using fewer plants. Therefore, when laboratory
facilities are already established MAS would be
recommended in breeding for MSV resistance.
Description
Keywords
Comparative Analysis, Breeding Maize Streak Virus, Resistance, Maize
Citation
Abalo, G., Tongoona, P., Derera, J., & Edema, R. (2009). A comparative analysis of conventional and marker‐assisted selection methods in breeding maize streak virus resistance in maize. Crop Science, 49(2), 509-520. doi: 10.2135/cropsci2008.03.0162