Susceptibility of Anopheles Mosquitoes to Insecticides Used In Busia and Tororo Districts, Eastern Uganda
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Research Square
Abstract
This study assessed the susceptibility of Anopheles mosquitoes to LLINs and IRS as vector control
interventions used in Tororo and Busia district. Samples of Anopheles mosquito larvae were collected from
various breeding grounds and reared in an insectary. Emerged adults were dissected under a stereoscopic
microscope and identified using standardized morphological keys after respective bioassays on used LLINs
and sprayed walls. A One-Way Analysis of Variance (ANOVA) was used to test for significant differences in the
mean composition of mosquito species across the study area, mean variations, mortalities by net and wall
type. Results showed that the Anopheles gambiae sensu lato revealed a higher mean of 78.33 ± 41.52,
compared to Anopheles funestus (16.42 ± 24.87) which was lonely found in Busia district. Out of four brands
of nets tested for insecticides susceptibility, 99% mortality was recorded for the insecticide concentration of 80
mg/m2 deltamethrin impregnated DAWA plus 2.0 nets. PermaNet 3.0 and Olyset recorded mortality rate of 95%
and 93% at chemical concentrations of 50 mg/m2 and 525 mg/m2 permethrin respectively. PermaNet 2.0
recorded 67% mortality at 55 mg/m2 permethrin. The average number of Anopheles mosquitoes were more
susceptible to 12.5 mg/m2 of Fludora fusion impregnated onto plastered painted walls at a rate of 100% as
compared to the other types of walls after 2 weeks (6.86 ± 4.07) and 1 month of chemical spray (6.69 ± 4.01)
varied insignificantly (t = 0.175, P = 0.861). Meanwhile, a disaggregated results by time of spray (T0 and T1)
and exposure periods, showed that plastered painted wall, had higher mortalities followed by Brick Plain and
Mud/Wattle wall substrates, respectively. This study has shown that vector behavior, biology and physiology
need consistent monitoring and surveillance for further entomological characterization and the need for
coming up with new vector control interventions.
Description
Keywords
Knocked down, Sentinel areas, Susceptibility, Oscillation, Plasmodium vector
Citation
Chemutai, F., Kisakye, J., Kabbale, F., & Egeru, A. (2022). Susceptibility of Anopheles Mosquitoes to Insecticides Used In Busia and Tororo Districts, Eastern Uganda. https://doi.org/10.21203/rs.3.rs-1638330/v1