Real time portable genome sequencing for global food security

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dc.contributor.authorBoykin, Laura
dc.contributor.authorGhalab, Ammar
dc.contributor.authorRossitto De Marchi, Bruno
dc.contributor.authorSavill, Anders
dc.contributor.authorWainaina, James M.
dc.contributor.authorKinene, Tonny
dc.contributor.authorLamb, Stephen
dc.contributor.authorRodrigues, Myriam
dc.contributor.authorKehoe, Monica
dc.contributor.authorNdunguru, Joseph
dc.contributor.authorTairo, Fred
dc.contributor.authorSseruwagi, Peter
dc.contributor.authorKayuki, Charles
dc.contributor.authorMark, Deogratius
dc.contributor.authorErasto, Joel
dc.contributor.authorBachwenkizi, Hilda
dc.contributor.authorAlicai, Titus
dc.contributor.authorOkao-Okuja, Geoffrey
dc.contributor.authorAbridrabo, Phillip
dc.contributor.authorOgwok, Emmanuel
dc.contributor.authorOsingada, John Francis
dc.contributor.authorAkono, Jimmy
dc.contributor.authorAteka, Elijah
dc.contributor.authorMuga, Brenda
dc.contributor.authorKiarie, Samuel
dc.date.accessioned2022-02-15T21:29:48Z
dc.date.available2022-02-15T21:29:48Z
dc.date.issued2018
dc.description.abstractCrop losses due to viral diseases and pests are major constraints on food security and income for millions of households in sub-Saharan Africa (SSA). Such losses can be reduced if plant diseases and pests are correctly diagnosed and identified early. Currently, accurate diagnosis for definitive identification of plant viruses and their vectors in SSA mostly relies on standard PCR and next generation sequencing technologies (NGS). However, it can take up to 6 months before results generated using these approaches are available. The long time taken to detect or identify viruses impedes quick, within-season decision-making necessary for early action, crop protection advice and disease control measures by farmers. This ultimately compounds the magnitude of crop losses and food shortages suffered by farmers. The MinION portable pocket DNA sequencer was used, to our knowledge globally for the first time, to sequence whole plant virus genomes. We used this technology to identify the begomoviruses causing the devastating cassava mosaic virus, which is ravaging smallholder farmers’ crops in sub-Saharan Africa.en_US
dc.identifier.citationBoykin L, Ghalab A, Rossitto De Marchi B et al. Real time portable genome sequencing for global food security [version 1; peer review: 1 approved, 1 approved with reservations] F1000Research 2018, 7:1101 https://doi.org/10.12688/f1000research.15507.1en_US
dc.identifier.urihttps://doi.org/10.12688/f1000research.15507.1
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2166
dc.language.isoenen_US
dc.publisherF1000 Researchen_US
dc.subjectCassavaen_US
dc.subjectUgandaen_US
dc.subjectKenyaen_US
dc.subjectTanzaniaen_US
dc.subjectNanoporeen_US
dc.subjectMinionen_US
dc.subjectSDG2en_US
dc.titleReal time portable genome sequencing for global food securityen_US
dc.typeArticleen_US
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