Dietary Restriction Induces a Stable Metabolic Obesity Phenotype in Drosophila Melanogaster
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Research Square
Abstract
Challenges associated with current nutritional models to induce obesity in Drosophila melanogaster
created a rationale for this study. The objective of the study was to investigate biochemical changes
associated with high-fat diet (HFD), high sucrose diet (HSD), and a protein-restricted diet (DR) to induce a
healthy metabolic obesity state. Drosophila melanogaster were fed to four experimental diets: regular food
(control), HFD, HSD, and DR, for four weeks. Peristaltic waves were measured on 3rd instar larvae, while
negative geotaxis, body mass, catalase activity; and total triglycerides, sterol, and protein were measured in
adult Drosophila melanogaster.
Results
DR produced a Drosophila melanogaster phenotype which had superior adaptive advantages than that
generated from HFD and HSD. HFD was the best phenotype during larval stages; however, locomotory, body
mass, triglyceride, sterol concentrations, and catalase activity were highest in the DR phenotype during
adulthood. High catalase activity and high triglyceride content demonstrated a balanced and healthy
metabolic obesity status than in other phenotypes in the adult stage. Evolutionary changes are responsible
for the selective advantage of the DR phenotype over the HFD phenotype. Prospective studies to guide
therapy and community behavior should place more emphasis on the DR phenotypes in Drosophila
melanogaster.
Description
Keywords
Metabolic obesity, Restrictive diets, Obesity, Triglyceride, Catalase, Drosophila melanogaster
Citation
Asiimwe, O. H., Sulaiman, S. O., Ochieng, J. J., Onanyang, D., Osuwat, L. O., Ndinawe, J. M., ... & Kasozi, K. I. (2021). Dietary Restriction Induces a Stable Metabolic Obesity Phenotype in Drosophila Melanogaster. https://orcid.org/0000-0002-5763-7964