Genetic Variability and Evolutionary Implications of RNA Silencing Suppressor Genes in RNA1 of Sweet Potato Chlorotic Stunt Virus Isolates Infecting Sweetpotato and Related Wild Species

dc.contributor.authorTugume, Arthur K.
dc.contributor.authorAmayo, Robert
dc.contributor.authorWeinheimer, Isabel
dc.contributor.authorMukasa, Settumba B.
dc.contributor.authorRubaihayo, Patrick R.
dc.contributor.authorValkonen, Jari P. T.
dc.date.accessioned2022-08-28T20:43:21Z
dc.date.available2022-08-28T20:43:21Z
dc.date.issued2013
dc.description.abstractThe bipartite single-stranded RNA genome of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus; Closteroviridae) encodes a Class 1 RNase III (RNase3), a putative hydrophobic protein (p7) and a 22-kDa protein (p22) from genes located in RNA1. RNase3 and p22 suppress RNA silencing, the basal antiviral defence mechanism in plants. RNase3 is sufficient to render sweetpotato (Ipomoea batatas) virus-susceptible and predisposes it to development of severe diseases following infection with unrelated virus. The incidence, strains and gene content of SPCSV infecting wild plant species have not been studied. Methodology/Principal Findings: Thirty SPCSV isolates were characterized from 10 wild Ipomoea species, Hewittia sublobata or Lepistemon owariensis (family Convolvulaceae) in Uganda and compared with 34 local SPCSV isolates infecting sweetpotatoes. All isolates belonged to the East African (EA) strain of SPCSV and contained RNase3 and p7, but p22 was not detected in six isolates. The three genes showed only limited genetic variability and the proteins were under purifying selection. SPCSV isolates lacking p22 synergized with Sweet potato feathery mottle virus (SPFMV, genus potyvirus; Potyviridae) and caused severe symptoms in co-infected sweetpotato plants. One SPCSV isolate enhanced accumulation of SPFMV, but no severe symptoms developed. A new whiteflytransmitted virus (KML33b) encoding an RNase3 homolog (<56% identity to SPCSV RNase3) able to suppresses sense-mediated RNA silencing was detected in I. sinensis. Conclusions/Significance: SPCSV isolates infecting wild species and sweetpotato in Uganda were genetically undifferentiated, suggesting inter-species transmission of SPCSV. Most isolates in Uganda contained p22, unlike SPCSV isolates characterized from other countries and continents. Enhanced accumulation of SPFMV and increased disease severity were found to be uncoupled phenotypic outcomes of RNase3-mediated viral synergism in sweetpotato. A second virus encoding an RNase3-like RNA silencing suppressor was detected. Overall, results provided many novel and important insights into evolutionary biology of SPCSV.en_US
dc.identifier.citationTugume AK, Amayo R, Weinheimer I, Mukasa SB, Rubaihayo PR, et al. (2013) Genetic Variability and Evolutionary Implications of RNA Silencing Suppressor Genes in RNA1 of Sweet Potato Chlorotic Stunt Virus Isolates Infecting Sweetpotato and Related Wild Species. PLoS ONE 8(11): e81479. doi:10.1371/journal.pone.0081479en_US
dc.identifier.other10.1371/journal.pone.0081479
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4479
dc.language.isoenen_US
dc.publisherPLoS ONEen_US
dc.subjectGenetic Variabilityen_US
dc.subjectRNA Silencing Suppressor Genesen_US
dc.subjectSweet Potato Chlorotic Stunt Virusen_US
dc.titleGenetic Variability and Evolutionary Implications of RNA Silencing Suppressor Genes in RNA1 of Sweet Potato Chlorotic Stunt Virus Isolates Infecting Sweetpotato and Related Wild Speciesen_US
dc.typeArticleen_US
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