The establishment of in vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum

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dc.contributor.authorSuganuma, Keisuke
dc.contributor.authorYamasaki, Shino
dc.contributor.authorInnocentia Molefe, Nthatisi
dc.contributor.authorMusinguzi, Peter Simon
dc.contributor.authorDavaasuren, Batdorj
dc.contributor.authorMossaad, Ehab
dc.contributor.authorNarantsatsral, Sandagdorj
dc.contributor.authorBattur, Banzragch
dc.contributor.authorBattsetseg, Badgar
dc.contributor.authorInoue, Noboru
dc.date.accessioned2023-02-09T17:07:37Z
dc.date.available2023-02-09T17:07:37Z
dc.date.issued2017
dc.description.abstractDourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T. equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T. equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T. equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent and an intracellular dehydrogenase activitybased colorimetric assay using WTS-8 tetrazolium salt (CCK-8 reagent) were used in order to examine the trypanocidal effects of each compound. In addition, the IC50 values of 4 reference trypanocidal compounds (pentamidine, diminazene, suramin and melarsomine) were evaluated and compared using established assays. The IC50 values of these reference compounds corresponded well to previous studies involving other strains of T. equiperdum. The luciferase assay would be suitable for the mass screening of chemical libraries against T. equiperdum because it allows for the simple and rapid-evaluation of the trypanocidal activities of test compounds, while a simple, inexpensive colorimetric assay will be applicable in developing countries for the evaluation of the drug sensitivity of epidemic trypanosome strains.en_US
dc.identifier.citationSuganuma, K., Yamasaki, S., Molefe, N. I., Musinguzi, P. S., Davaasuren, B., Mossaad, E., ... & Inoue, N. (2017). The establishment of in vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum. International Journal for Parasitology: Drugs and Drug Resistance, 7(2), 200-205. http://dx.doi.org/10.1016/j.ijpddr.2017.04.002en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.ijpddr.2017.04.002
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7706
dc.language.isoenen_US
dc.publisherDrugs and Drug Resistanceen_US
dc.subjectColorimetric assayen_US
dc.subjectDrug screening systemen_US
dc.subjectLiquid cultureen_US
dc.subjectLuciferase assayen_US
dc.subjectTrypanosoma equiperdumen_US
dc.titleThe establishment of in vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdumen_US
dc.typeArticleen_US
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