The evaluation of GM6-based ELISA and ICT as diagnostic methods on a Mongolian farm with an outbreak of non-tsetse transmitted horse trypanosomosis
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Veterinary Parasitology
Abstract
Trypanosoma equiperdum, which is the etiological agent of dourine, spreads
through sexual intercourse in equines. Dourine (T. equiperdum) has been
reported in Mongolia, where it is considered an economically important disease
of horses. T. evansi has also been reported in Mongolian domestic animals. The
objective of this study was to evaluate the potential application of recombinant T.
evansi GM6 (rTeGM6-4r)-based diagnostic methods on a farm with an outbreak
of non-tsetse transmitted horse trypanosomosis. Ninety-seven percent homology was found between the amino acid
sequences of T. equiperdum GM6 and the GM6 of another Trypanozoon, which
also shared the same cellular localization. This finding suggests the utility of
rTeGM6-4r-based serodiagnostic methods for epidemiological studies and the
diagnosis of both surra and dourine in Equidae.
Fifty blood samples were examined from a herd of horses. The diagnostic
value of an rTeGM6-4r-based ELISA and an rTeGM6-4r-based
immunochromatographic test (ICT) were measured in comparison to a T. evansi
crude antigen-based ELISA, which is a diagnostic method recommended by the
OIE. However, this is not a perfect diagnostic method for trypanosomosis.
Positive serum samples were detected in 46%, 42% and 28% of the tested
horses using an rTeGM6-4r-based ELISA, crude antigen-based ELISA and
rTeGM6-4r-based ICT, respectively. The sensitivity of rTeGM6-based ELISA was
81%, the specificity was 79%, and the agreement was moderate. We conclude
that rTeGM6-4r-based ELISA and ICT represent alternative options for baseline epidemiological studies and the on-site diagnosis of horse trypanosomoses in
the field, respectively.
Description
Keywords
Dourine, ELISA, Immunochromatographic test, Mongolia, Outbreak
Citation
Davaasuren, Batdorj, Amgalanbaatar, Tovuu, Musinguzi, Simon Peter, Suganuma, Keisuke, Otgonsuren, Davaajav, Mossaad, Ehab, Narantsatsral, Sandagdorj, Battur, Banzragch, Battsetseg, Badgar, Xuan, Xuenan, Inoue, Noboru, The evaluation of GM6-based ELISA and ICT as diagnostic methods on a Mongolian farm with an outbreak of non-tsetse transmitted horse trypanosomosis.Veterinary Parasitology http://dx.doi.org/10.1016/j.vetpar.2017.07.036