Evaluation of three protocols for direct susceptibility testing for gram negative-Enterobacteriaceae from patient samples in Uganda...
| dc.contributor.author | Aruhomukama, Dickson | |
| dc.contributor.author | Magiidu, Walusimbi Talemwa | |
| dc.contributor.author | Katende, George | |
| dc.contributor.author | Ebwongu, Robert Innocent | |
| dc.contributor.author | Bulafu, Douglas | |
| dc.contributor.author | Kasolo, Rajab | |
| dc.contributor.author | Nakabuye, Hellen | |
| dc.contributor.author | Musoke, David | |
| dc.contributor.author | Asiimwe, Benon | |
| dc.date.accessioned | 2024-02-07T08:33:34Z | |
| dc.date.available | 2024-02-07T08:33:34Z | |
| dc.date.issued | 2024-02-01 | |
| dc.description | We are grateful for the technical and administrative assistance provided by the staff at the Clinical Microbiology laboratory within the Department of Microbiology, College of Health Sciences, Makerere University, and the staff at the Makerere University Research and Innovations Fund Secretariat, Makerere University. | en_US |
| dc.description.abstract | In Uganda, the challenge of generating and timely reporting essential antimicrobial resistance (AMR) data has led to overreliance on empirical antibiotic therapy, exacerbating the AMR crisis. To address this issue, this study aimed to adapt a one-step AMR testing protocol alongside an SMS (Short Message Service) result relay system (SRRS), with the potential to reduce the turnaround time for AMR testing and result communication from 4 days or more to 1 day in Ugandan clinical microbiology laboratories. Out of the 377 samples examined, 54 isolates were obtained. Notably, E. coli (61%) and K. pneumoniae (33%) were the most frequently identified, majority testing positive for ESBL. Evaluation of three AMR testing protocols revealed varying sensitivity and specificity, with Protocol A (ChromID ESBL-based) demonstrating high sensitivity (100%) but no calculable specificity, Protocol B (ceftazidime-based) showing high sensitivity (100%) and relatively low specificity (7.1%), and Protocol C (cefotaxime-based) exhibiting high sensitivity (97.8%) but no calculable specificity. ESBL positivity strongly correlated with resistance to specific antibiotics, including cefotaxime, ampicillin, and aztreonam (100%), cefuroxime (96%), ceftriaxone (93%), and trimethoprim sulfamethoxazole (87%). The potential of integrating an SRRS underscored the crucial role this could have in enabling efficient healthcare communication in AMR management. This study underscores the substantial potential of the tested protocols for accurately detecting ESBL production in clinical samples, potentially, providing a critical foundation for predicting and reporting AMR patterns. Although considerations related to specificity warrant careful assessment before widespread clinical adoption. | en_US |
| dc.description.sponsorship | This work was supported by the Government of Uganda through Makerere University Research and Innovations Fund (MakRIF) under MAKRIF-4. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en_US |
| dc.identifier.citation | Aruhomukama, Dickson, Walusimbi Talemwa Magiidu, George Katende, et al. 'Evaluation of Three Protocols for Direct Susceptibility Testing for Gram Negative-Enterobacteriaceae from Patient Samples in Uganda with SMS Reporting', Scientific Reports, vol. 14/no. 1, (2024), pp. 2730. | en_US |
| dc.identifier.issn | EISSN 2045-2322 | |
| dc.identifier.uri | https://nru.uncst.go.ug/handle/123456789/9399 | |
| dc.language.iso | en | en_US |
| dc.publisher | Nature Publishing Group | en_US |
| dc.subject | one-step AMR testing protocol; SMS (Short Message Service); Uganda | en_US |
| dc.title | Evaluation of three protocols for direct susceptibility testing for gram negative-Enterobacteriaceae from patient samples in Uganda... | en_US |
| dc.type | Article | en_US |