In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)

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dc.contributor.authorButtibwa, Mary
dc.contributor.authorKawuki, Robert S.
dc.contributor.authorTugume, Arthur K.
dc.contributor.authorAkol, Jacinta
dc.contributor.authorMagambo, Stephen
dc.contributor.authorApio, Hellen
dc.contributor.authorHeberle-Bors, Erwin
dc.contributor.authorWedzony, Maria
dc.contributor.authorCeballos, Hernán
dc.contributor.authorHershey, Clair
dc.contributor.authorBaguma, Yona
dc.date.accessioned2022-03-07T12:52:26Z
dc.date.available2022-03-07T12:52:26Z
dc.date.issued2015
dc.description.abstractCassava is a highly heterozygous species; hence, current methods used in classical cassava breeding cannot match the urgent need to high yielding varieties. Recently, progress was made through androgenesis and gynogenesis as pathways for raising doubled cassava haploid lines to overcome problems associated with cassava’s inherent reproductive biology, but these efforts were limited (no candidate cassava plantlets were regenerated). For the first time, this study shows that pollen irradiation coupled with self-pollination and embryo rescue regenerated 62 candidate cassava plantlets. Plants of an elite cassava variety, Nase14, served as a mother plant and as the pollen donor for the irradiation. Irradiation dosages of 50 to 250 Gray studied across five pollination events and 300 or 500 Gray in one pollination event caused a reduction in pollen germination up to 67.0%. By 15 days after pollination (DAP) with irradiated pollen, up to 89.7% of the pollinated flowers had aborted. By embryo rescue time (42 DAP), significant differences were observed in number of fruits, seeds and embryos generated, with the non-irradiated pollen treatments having significantly higher numbers. Sixteen (16) heterozygous SSR markers in the parent and ploidy analysis showed that none of the regenerated plants was haploid or homozygous. However, the plantlets resulting from pollination with non-irradiated pollen had 56.2% homozygous loci, while progeny derived from irradiated treatments had frequencies of homozygous loci between 28.1 and 55.0%. This is the first time to use irradiated pollen in cassava as a pathway to generate candidate plantlets as an initial step in double haploid production.en_US
dc.identifier.citationButtibwa, M., Kawuki, R. S., Tugume, A. K., Akol, J., Magambo, S., Apio, H., ... & Baguma, Y. (2015). In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz). African Journal of Biotechnology, 14(27), 2191-2201. DOI:10.5897/AJB2015.14577en_US
dc.identifier.issn1684-5315
dc.identifier.other10.5897/AJB2015.14577
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2482
dc.language.isoenen_US
dc.publisherAfrican Journal of Biotechnologyen_US
dc.subjectCassavaen_US
dc.subjectDoubled haploidsen_US
dc.subjectEmbryo rescueen_US
dc.subjectPlant regenerationen_US
dc.subjectPollen germinationen_US
dc.subjectPollen irradiation.en_US
dc.titleIn vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)en_US
dc.typeArticleen_US
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