Dynamics of Plasmodium falciparum alleles in children with normal haemoglobin and with sickle cell trait in western Uganda

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dc.contributor.authorKiwanuka, Gertrude N.
dc.contributor.authorJoshib, Hema
dc.contributor.authorIsharazaa, William K.
dc.contributor.authorEschrichc, Klaus
dc.date.accessioned2022-03-07T11:07:51Z
dc.date.available2022-03-07T11:07:51Z
dc.date.issued2009
dc.description.abstractWe describe the diversity of Plasmodium falciparum populations in western Uganda and assess the role that asymptomatic malaria carriers with sickle cell trait (HbAS) may be playing on the Plasmodium population structure. We genotyped P. falciparum in 291 samples using merozoite surface protein (MSP) 1 and 2 loci. Extensive genetic diversity was detected among symptomatic children in Mbarara (20 MSP1 alleles; 31 MSP2 alleles) and Kagando, Kasese (19 MSP1 alleles; 30 MSP2 alleles). Multiplicity of infection (MOI) was significantly higher in Kagando, Kasese than in Mbarara, with 2.7 and 2.1 genotypes/PCR positive sample with MSP2 marker, respectively. Similar strains were circulating in the two sites; however, a few strains specific to individual sites were observed. Prevalence of HbAS was 36% (12/33) among asymptomatic children in Kisinga sub-county, Kasese. In asymptomatic children, MOI was age-dependent and higher in HbAS carriers than HbAA, suggesting that HbAS carriers harbour a wider range of P. falciparum genotypes. Sickle cell trait may influence rapid acquisition of premunition by creating a reservoir of variant parasite strains in the host. The high level of genetic diversity demonstrated here shows that even in areas with low or seasonal transmission, high levels of parasite polymorphism can occur.en_US
dc.identifier.citationKiwanuka, G. N., Joshi, H., Isharaza, W. K., & Eschrich, K. (2009). Dynamics of Plasmodium falciparum alleles in children with normal haemoglobin and with sickle cell trait in western Uganda. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103(1), 87-94. doi:10.1016/j.trstmh.2008.07.023en_US
dc.identifier.other10.1016/j.trstmh.2008.07.023
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2455
dc.language.isoenen_US
dc.publisherTransactions of the Royal Society of Tropical Medicine and Hygieneen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectMerozoite surface proteinen_US
dc.subjectAllelesen_US
dc.subjectHaemoglobinen_US
dc.subjectChildrenen_US
dc.subjectUgandaen_US
dc.titleDynamics of Plasmodium falciparum alleles in children with normal haemoglobin and with sickle cell trait in western Ugandaen_US
dc.typeArticleen_US
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