Identification of Flea Blood Meals Using Multiplexed Real-Time Polymerase Chain Reaction Targeting Mitochondrial Gene Fragments

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dc.contributor.authorWoods, Michael E.
dc.contributor.authorMontenieri, John A.
dc.contributor.authorEisen, Rebecca J.
dc.contributor.authorZeidner, Nordin S.
dc.contributor.authorBorchert, Jeff N.
dc.contributor.authorLaudisoit, Anne
dc.contributor.authorBabi, Nackson
dc.contributor.authorAtiku, Linda A.
dc.contributor.authorEnscore, Russell E.
dc.contributor.authorGage, Kenneth L.
dc.date.accessioned2021-12-16T06:49:02Z
dc.date.available2021-12-16T06:49:02Z
dc.date.issued2009
dc.description.abstractis found in the West Nile region of Uganda and Democratic Republic of the Congo where flea vectors are often found inhabiting homes. We have developed a multiplexed, real-time polymerase chain reaction assay targeting mitochondrial genes that is capable of detecting blood meal sources in fleas collected off-host in East Africa. Laboratory tests showed that the assay is specific for the intended targets and has a detection limit below one picogram of DNA. Testing of wild-caught fleas from the Democratic Republic of Congo suggests that humans are at significant risk from flea-borne disease and implicates domestic animals including cats, chickens, and the black rat as potential sources of human exposure to fleas and flea-borne diseases. Future application of the assay will help us better define the ecology of plague in East Africa to implement effective control measures to combat the spread of disease.en_US
dc.identifier.citationWoods, M. E., Montenieri, J. A., Eisen, R. J., Zeidner, N. S., Borchert, J. N., Laudisoit, A., ... & Gage, K. L. (2009). Identification of flea blood meals using multiplexed real-time polymerase chain reaction targeting mitochondrial gene fragments. The American journal of tropical medicine and hygiene, 80(6), 998-1003. DOI: https://doi.org/10.4269/ajtmh.2009.80.998en_US
dc.identifier.urihttps://doi.org/10.4269/ajtmh.2009.80.998
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/690
dc.language.isoenen_US
dc.publisherThe American journal of tropical medicine and hygieneen_US
dc.relation.ispartofseriesThe American journal of tropical medicine and hygiene;80(6)
dc.subjectHuman plagueen_US
dc.subjectMultiplexed Real-Time Polymerase Chainen_US
dc.titleIdentification of Flea Blood Meals Using Multiplexed Real-Time Polymerase Chain Reaction Targeting Mitochondrial Gene Fragmentsen_US
dc.typeArticleen_US
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