Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant

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dc.contributor.authorOwor, Betty E.
dc.contributor.authorMartin, Darren P.
dc.contributor.authorShepherd, Dionne N.
dc.contributor.authorEdema, Richard
dc.contributor.authorMonjane, Ade´ rito L.
dc.contributor.authorRybicki, Edward P.
dc.contributor.authorThomson, Jennifer A.
dc.contributor.authorVarsani, Arvind
dc.date.accessioned2022-06-12T18:46:46Z
dc.date.available2022-06-12T18:46:46Z
dc.date.issued2007
dc.description.abstractMaize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants – MSV subtypes A1–A6 – causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR–restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for .60% of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species.en_US
dc.identifier.citationOwor, BE, Martin, DP, Shepherd, DN, Edema, R., Monjane, AL, Rybicki, EP, ... & Varsani, A. (2007). Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. Journal of general virology , 88 (11), 3154-3165. DOI 10.1099/vir.0.83144-0en_US
dc.identifier.other10.1099/vir.0.83144-0
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3930
dc.language.isoenen_US
dc.publisherJournal of general virologyen_US
dc.subjectMaize streak virusen_US
dc.subjectUgandaen_US
dc.subjectGenetic analysisen_US
dc.subjectRecombinant varianten_US
dc.titleGenetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant varianten_US
dc.typeArticleen_US
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