Bacterial and Fungal Contaminants in Mycobacterium tuberculosis Cultures at National Tuberculosis Referral Laboratory-Kampala
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Microbiology Research Journal International
Abstract
Aim: This study was aimed at identification of fungal and bacterial contaminants in Tuberculosis cultures at the Uganda National Reference Laboratory (NTRL).
Methods: The cultures were previously grown on Lowenstein-Jensen (LJ) medium in bottle slants. The method used involved conventional culture methods and biochemical characterization of the contaminants. Bacterial cultures were carried out in both aerobic and carbon dioxide incubators where required and to the respective temperatures of the organisms. Growth was observed between 24-48 hours for bacterial and up to 72 hours for yeast contaminants. Cultures on SDA were also
monitored for three weeks for possible growth of filamentous fungi. Results: The identified bacteria mostly included; S. pyogenes, S. aureus, E. coli, P. aeruginosa, K. pneumoniae, and least isolated were; S. marcescens, H. influenza, S. pneumoniae and B. subtilis Fungal contaminants were A. fumigatus and C. albicans.
Conclusion: Based on this study a relatively increased level of contamination was noted. We
recommend that high level of aseptic techniques be maintained in addition to the current standards
in order to reduce the level of bacterial and fungal contaminants in TB cultures so as to improve the
detection rate of Mycobacteria tuberculosis by culture method.
Description
Keywords
Bacteria, Contaminants, Mycobacterium tuberculosis
Citation
Vianney, T. J., Esther, K., & Stanley, I. J. (2017). Bacterial and fungal contaminants in mycobacterium tuberculosis cultures at national tuberculosis referral laboratory-Kampala. Microbiology Research Journal International, 1-8.DOI: 10.9734/MRJI/2017/32801