Multicountry validation of SAMBA - a novel molecular point-of-care test for HIV-1 detection in resource-limited setting

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dc.contributor.authorFowler, G. Mary
dc.date.accessioned2022-02-14T08:29:37Z
dc.date.available2022-02-14T08:29:37Z
dc.date.issued2017
dc.description.abstractIntroduction: Early diagnosis of HIV-1 infection and the prompt initiation of antiretroviral therapy are critical to achieving a reduction in the morbidity and mortality of infected infants. The Simple AMplification-Based Assay (SAMBA) HIV-1 Qual Whole Blood Test was developed specifically for early infant diagnosis and prevention of mother-to-child transmission programs implemented at the point-of-care in resource-limited settings. Methods: We have evaluated the performance of this test run on the SAMBA I semiautomated platform with fresh whole blood specimens collected from 202 adults and 745 infants in Kenya, Uganda, and Zimbabwe. Results were compared with those obtained with the Roche COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) HIV-1 assay as performed with fresh whole blood or dried blood spots of the same subjects, and discrepancies were resolved with alternative assays. Results: The performance of the SAMBA and CAP/CTM assays evaluated at 5 laboratories in the 3 countries was similar for both adult and infant samples. The clinical sensitivity, specificity, positive predictive value, and negative predictive value for the SAMBA test were 100%, 99.2%, 98.7%, and 100%, respectively, with adult samples, and 98.5%, 99.8%, 99.7%, and 98.8%, respectively, with infant samples. Discussion: Our data suggest that the SAMBA HIV-1 Qual Whole Blood Test would be effective for early diagnosis of HIV-1 infection in infants at point-of-care settings in sub-Saharan Africa.en_US
dc.identifier.citationOndiek, J., Namukaya, Z., Mtapuri-Zinyowera, S., Balkan, S., Elbireer, A., Lumb, I. U., ... & Lee, H. (2017). Multicountry validation of SAMBA-A novel molecular point-of-care test for HIV-1 detection in resource-limited setting. JAIDS Journal of Acquired Immune Deficiency Syndromes, 76(2), e52-e57.en_US
dc.identifier.other10.1097/QAI.0000000000001476
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2104
dc.language.isoenen_US
dc.publisherWolters Kluwer Health, Incen_US
dc.subjectHIV-1en_US
dc.subjectPoint-of-careen_US
dc.subjectEarly infant diagnosisen_US
dc.subjectNucleic acid testen_US
dc.titleMulticountry validation of SAMBA - a novel molecular point-of-care test for HIV-1 detection in resource-limited settingen_US
dc.typeArticleen_US
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