The Lys-76-Thr mutation in WC/Wand chloroquine resistance in Plasmodium falciparum isolates from Uganda

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dc.contributor.authorKyosiimire-Lugemwa, J.
dc.contributor.authorNalunkuma-Kazibwe, A.J.
dc.contributor.authorMujuzi, G.
dc.contributor.authorMulindwa, H.
dc.contributor.authorTalisuna, A.
dc.contributor.authorEgwang, T.G.
dc.date.accessioned2022-05-28T19:10:00Z
dc.date.available2022-05-28T19:10:00Z
dc.date.issued2002
dc.description.abstractRecent molecular studies of chloroquine (CQ) resistance of Plasmodium falciparum have demonstrated an association between a mutation in the PƒCRT gene and CQ resistance. We identified wild type and mutant alleles of the PƒCRT codon 76 in baseline pre-CQ treatment P. falciparum isolates collected during 1999 and investigated their relationship to CQ efficacy in 3 different sites with different levels of CQ parasite resistance in Uganda. Of 32 isolates from Mulago Hospital, all were mutant (100%), while of 45 isolates from Tororo, 5 (11%) were mixed wild type and mutant and 40 (89%) were mutants only. Of 41 isolates from Apac, 13 (32%) were mixed wild type and mutant whereas 28 (68%) were mutants only. The finding of 100% prevalence of the Thr-76 mutant allele in all isolates at the 3 sites was remarkable. We found no association between the presence of Thr-76 mutation and treatment outcome at all the sites. However, the prevalence of the wild-type Lys-76 allele was higher in Apac, an area with lower CQ parasite resistance, compared to Tororo and Mulago which have relatively higher CQ parasite resistance. The Thr-76 allele as a marker of CQ resistance is probably useful in regions where the allele frequency has not yet plateaued.en_US
dc.identifier.citationKyosiimire-Lugemwa, J., Nalunkuma-Kazibwe, A. J., Mujuzi, G., Mulindwa, H., Talisuna, A., & Egwang, T. G. (2002). The Lys-76-Thr mutation in PfCRTand chloroquine resistance in Plasmodium falciparum isolates from Uganda. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96(1), 91-95.https://doi.org/10.1016/S0035-9203(02)90252-Xen_US
dc.identifier.issn0035-9203
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3617
dc.language.isoenen_US
dc.publisherTransactions of the Royal Society of Tropical Medicine and Hygieneen_US
dc.subjectPlasmodium falciparumgenetic, analysisgene mutation,PƒCRT,drug resistance,chloroquine,Ugandaen_US
dc.titleThe Lys-76-Thr mutation in WC/Wand chloroquine resistance in Plasmodium falciparum isolates from Ugandaen_US
dc.typeArticleen_US
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