Assessment of replicate numbers for titrating avian influenza virus using dose-response models

Simple item page
dc.contributor.authorSpackman, Erica
dc.contributor.authorMalladi, Sasidhar
dc.contributor.authorSsematimba, Amos
dc.contributor.authorStephens, Christopher B.
dc.date.accessioned2023-01-04T20:17:13Z
dc.date.available2023-01-04T20:17:13Z
dc.date.issued2019
dc.description.abstractEmbryonating chicken eggs (ECEs) are among the most sensitive laboratory host systems for avian influenza virus (AIV) titration, but ECEs are expensive and require space for storage and incubation. Therefore, reducing ECE use would conserve resources. We utilized statistical modeling to evaluate the accuracy and precision of AIV titration with 3 instead of 5 ECEs for each dilution by the Reed–Muench method for 50% endpoint calculation. Beta-Poisson and exponential doseresponse models were used in a simulation study to evaluate observations from actual titration data from 18 AIV isolates. The reproducibility among replicates of a titration was evaluated with one AIV isolate titrated in 3 replicates with the beta-Poisson, exponential, and Weibull dose-response models. The standard deviation (SD) of the error between input and estimated virus titers was estimated with Monte Carlo simulations using the fitted dose-response models. Good fit was observed with all models that were utilized. Reducing the number of ECEs per dilution from 5 to 3 resulted in the width of the 95% confidence interval increasing from ±0.64 to ±0.75 log 10 50% ECE infectious doses (EID 50 ) and the SD of the error increased by 0.03 log 10 EID 50 . Our study suggests that using fewer ECEs per dilution is a viable approach that will allow laboratories to reduce costs and improve efficiency.en_US
dc.identifier.citationSpackman, E., Malladi, S., Ssematimba, A., & Stephens, C. B. (2019). Assessment of replicate numbers for titrating avian influenza virus using dose-response models. Journal of Veterinary Diagnostic Investigation, 31(4), 616-619. https://doi.org/10.1177/10406387198538en_US
dc.identifier.otherhttps://doi.org/10.1177/10406387198538
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/6811
dc.language.isoenen_US
dc.publisherJournal of Veterinary Diagnostic Investigationen_US
dc.subjectAvian influenza virusen_US
dc.subjectEmbryonating chicken egg titrationen_US
dc.subjectVirus quantificationen_US
dc.titleAssessment of replicate numbers for titrating avian influenza virus using dose-response modelsen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Assessment of replicate numbers for titrating.pdf
Size:
80.22 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences