Expression of a rice chitinase gene in transgenic banana (‘Gros Michel’, AAA genome group) confers resistance to black leaf streak disease
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Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Transgenic research
Abstract
Transgenic banana (Musa acuminata
‘Gros Michel’) integrating either of two rice chitinase
genes was generated and its resistance to Black Leaf
Streak disease caused by the fungus Mycosphaerella
fijiensis was tested using a leaf disk bioassay. PCR
screening indicated the presence of the hpt selectable
marker gene in more than 90 % of the lines tested,
whereas more than three quarters of the lines
contained the linked rice chitinase gene resulting in a
co-transformation frequency of at least 71.4 %. Further,
a unique stable integration of the transgenes in
each line revealed some false negative PCR results and
the expected co-transformation frequency of 100 %.
Gabriella Kova´cs, La´szlo´ Sa´gi contributed equally to this work.
Description
Keywords
Black leaf streak disease Leaf disk assay Musa spp. Mycosphaerella fijiensis Transgenic banana
Citation
Kovács, G., Sági, L., Jacon, G., Arinaitwe, G., Busogoro, J. P., Thiry, E., ... & Remy, S. (2013). Expression of a rice chitinase gene in transgenic banana (‘Gros Michel’, AAA genome group) confers resistance to black leaf streak disease. Transgenic research, 22(1), 117-130.(doi:10.1007/s11248-012-9631-1)