A Pepsin-Revealed Material Possibly Related to Chromosomal Banding
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Date
1978
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Chromosoma
Abstract
The enzymes pepsin, c~-chymotrypsin, trypsin, RNase and DNase
were applied to preparations of human metaphase chromosomes before
staining to study whether dissociable materials related to the formation
of G-, Q- and C-bands would be seen. Treatment with active pepsin but
not the other enzymes revealed material with ribonucleo-protein properties
which dissociated from the chromosomes and formed a halo. - Lateral
extensions from the chromatids stretched to the rim of the halo and appeared
at positions corresponding to G-bands. A G-band may be defined as a
ring of stable chromatid-matrix binding at positions where the chromatids
coil to form lateral extensions.
Description
Keywords
Pepsin-Revealed Material, Chromosomal Banding
Citation
Byarugaba, W. (1978). A pepsin-revealed material possibly related to chromosomal banding. Chromosoma, 67(3), 275-283.