Immune correlates of HIV exposure without infection in foreskins of men from Rakai, Uganda

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dc.contributor.authorProdger, J. L.
dc.contributor.authorHirbod, T.
dc.contributor.authorKigozi, G.
dc.contributor.authorNalugoda, F.
dc.contributor.authorReynolds, S. J.
dc.contributor.authorGaliwango, R.
dc.contributor.authorShahabi, K.
dc.contributor.authorSerwadda, D.
dc.contributor.authorWawer, M.J.
dc.contributor.authorGray, R. H.
dc.contributor.authorKaul, R.
dc.date.accessioned2022-01-24T22:21:11Z
dc.date.available2022-01-24T22:21:11Z
dc.date.issued2014
dc.description.abstractHuman immunodeficiency virus (HIV) susceptibility is heterogenous, with some HIV-exposed but seronegative (HESN) individuals remaining uninfected despite repeated exposure. Previous studies in the cervix have shown that reduced HIV susceptibility may be mediated by immune alterations in the genital mucosa. However, immune correlates of HIV exposure without infection have not been investigated in the foreskin. We collected sub-preputial swabs and foreskin tissue from HESN (n¼20) and unexposed control (n¼57) men undergoing elective circumcision. Blinded investigators assayed swabs for HIV-neutralizing IgA, innate antimicrobial peptides, and cytokine levels. Functional T-cell subsets from foreskin tissue were assessed by flow cytometry. HESN foreskins had elevated a-defensins (3,027 vs. 1,795 pgml 1,P¼0.011) and HIV-neutralizing IgA(50.0 vs. 13.5%ofmen,P¼0.019).Foreskin tissue from HESN men contained a higherdensityofCD3 T cells (151.9 vs. 69.9 cellsmm 2, P¼0.018), but a lower proportion of these was Th17 cells (6.12 vs. 8.04%of CD4 Tcells, P¼0.007), and fewer produced tumor necrosis factor a (TNFa) (34.3 vs. 41.8% of CD4 Tcells, P¼0.037; 36.9 vs. 45.7%of CD8 T cells, P¼0.004). A decrease in the relative abundance of susceptible CD4 T cells and local TNFa production, in combination with HIV-neutralizing IgA and a defensins, may represent a protective immune milieu at a site of HIV exposure.en_US
dc.identifier.citationProdger, J. L., Hirbod, T., Kigozi, G., Nalugoda, F., Reynolds, S. J., Galiwango, R., ... & Kaul, R. (2014). Immune correlates of HIV exposure without infection in foreskins of men from Rakai, Uganda. Mucosal immunology, 7(3), 634-644.en_US
dc.identifier.urihttps://www.nature.com/articles/mi201383
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1538
dc.language.isoenen_US
dc.publisherMucosal immunologyen_US
dc.subjectImmune correlatesen_US
dc.subjectHIVen_US
dc.subjectInfectionen_US
dc.subjectForeskins of menen_US
dc.subjectUgandaen_US
dc.titleImmune correlates of HIV exposure without infection in foreskins of men from Rakai, Ugandaen_US
dc.typeArticleen_US
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