Comparative detection of African swine fever virus by loop-mediated isothermal amplification assay and polymerase chain reaction in domestic pigs in Uganda

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dc.contributor.authorAtuhaire, David Kalenzi
dc.contributor.authorAfayoa, Mathias
dc.contributor.authorKatiti, Dianah
dc.contributor.authorMwiine, Frank Norbert
dc.contributor.authorNanteza, Ann
dc.contributor.authorMugasa, Claire Mack
dc.contributor.authorMatovu, Enock
dc.contributor.authorOlaho-Mukani, William
dc.contributor.authorOjok, Lonzy
dc.date.accessioned2023-03-06T20:25:38Z
dc.date.available2023-03-06T20:25:38Z
dc.date.issued2014
dc.description.abstractAfrican swine fever (ASF) is a contagious viral disease, which can cause up to 100% mortality among domestic pigs. Pig production is growing rapidly in Uganda among East African countries and is not only a source of food but also an important income for many people living in the rural areas. Field diagnosis of ASF depends only on clinical signs and has to be confirmed in the laboratory since the clinical signs are not pathognomonic. Diagnostic techniques for ASF are focused on serological tests for detection of antigen and antibody, genomic DNA detection by polymerase chain reaction (PCR), and on virus isolation and localization in clinical samples. There have been many recent reports of ASF outbreaks in Uganda yet laboratory diagnosis is limited due to the high cost and expertise required. This work reports the evaluation and application of a loop-mediated isothermal amplification (LAMP) test for detecting African swine fever virus (ASFV) DNA based on the topoisomerase II gene. Thirty (30) tissue samples obtained from suspected ASF outbreaks were collected from different regions of Uganda. The tissue samples were found to have lesions consistent with ASF. One hundred and eighty eight (188) additional blood samples were obtained from the abattoir and field surveillance. Six primers targeting the topoisomerase II gene were used. The sensitivity and specificity of LAMP and OIE recommended diagnostic PCR were compared. The LAMP assay is rapid with results obtained within 1 h (45-60 min). The sensitivity of LAMP for the detection of ASFV was 100% (95% CI: 91.78-100) while the specificity was 44% (95% CI: 36.52-51.69). The Kappa statistic for level of agreement between PCR and LAMP test in the detection of ASFV was 23.7% (95% CI: 16.42-30.91). This Kappa value indicated a fair agreement between the two assays. No cross reaction was observed with Porcine circovirus type 2virus and E. coli isolated from pigs in Uganda. This is the first study evaluating and applying the LAMP assay in the detection of ASF in domestic pigs in Uganda. The LAMP assay was found to be more sensitive than PCR. Due to its simplicity, sensitivity and specificity, the LAMP assay has the potential for use in the diagnosis and routine surveillance of ASF in Uganda.en_US
dc.identifier.citationAtuhaire, D. K., Afayoa, M., Ochwo, S., Katiti, D., Mwiine, F. N., Nanteza, A., ... & Ojok, L. (2014). Comparative detection of African swine fever virus by loop-mediated isothermal amplification assay and polymerase chain reaction in domestic pigs in Uganda. African Journal of Microbiology Research, 8(23), 2322-2328.DOI: 10.5897/AJMR2014.6848en_US
dc.identifier.issn1996-0808
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8095
dc.language.isoenen_US
dc.publisherAfrican Journal of Microbiology Researchen_US
dc.subjectAfrican swine fever virusen_US
dc.subjecttopoisomerase II gene.en_US
dc.subjectloop-mediated isothermal amplification (LAMP)en_US
dc.titleComparative detection of African swine fever virus by loop-mediated isothermal amplification assay and polymerase chain reaction in domestic pigs in Ugandaen_US
dc.typeArticleen_US
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