• Login
    View Item 
    •   NRU
    • Journal Publications
    • Agricultural and Veterinary Sciences
    • Agricultural and Veterinary Sciences
    • View Item
    •   NRU
    • Journal Publications
    • Agricultural and Veterinary Sciences
    • Agricultural and Veterinary Sciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Genetic Characterisation and Diversity Assessment of Potato Genotypes Using SSR Markers

    Thumbnail
    View/Open
    Article (355.1Kb)
    Date
    2017
    Author
    Namugga, Prossy
    Sibiya, Julia
    Melis, Rob
    Barekye, Alex
    Metadata
    Show full item record
    Abstract
    Information on diversity of genetic materials is vital for choosing parents in a breeding program. The objective of the study was to determine the pattern and level of genetic diversity among the selected 20 tetraploid potato genotypes using 16 SSR markers to identify suitable parents for breeding purposes. The microsatellites showed considerable variation among genotypes and sixty four alleles were amplified by the 16 primer pairs. The number of polymorphic alleles per locus ranged from 2 to 8 with an average of 3.9. The highest number of null alleles was observed was six for genotype Nakpot1. The overall size of the amplified product varied from 48 bp (marker STI0023) to 309 bp (marker STM5121). PIC values ranged from 0.0948 to 0.7832, with an average of 0.4307 per locus. Heterozygosity values ranged from 0.0997 to 0.805 with an average of 0.466919. Significant positive linear correlations were observed between PIC values and number of alleles (r = 0.905); and heterozygosity and number of alleles (r = 0.8659) at p < 0.001. Cluster analysis separated the genotypes into three different groups. The genetic distance between clones ranged from 1 to 5.7. Cruza had the highest genetic distance while the shortest genetic distance was observed between 396026.103 and 396034.104. The microsatellites used in this study provided useful information regarding the variability of the tested genotypes and their selection for breeding purposes.
    URI
    https://doi.org/10.5539/jas.v9n8p99
    https://nru.uncst.go.ug/xmlui/handle/123456789/2256
    Collections
    • Agricultural and Veterinary Sciences [1451]

    Research Dissemination Platform copyright © since 2021  UNCST
    Contact Us | Send Feedback
    Partners
     

     

    Browse

    All of NRU
    Communities & CollectionsBy Issue DateAuthorsTitlesSubjects
    This Collection
    By Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Research Dissemination Platform copyright © since 2021  UNCST
    Contact Us | Send Feedback
    Partners