Diversity and Antimicrobial Resistance Genotypes in Non-Typhoidal Salmonella Isolates from Poultry Farms in Uganda
Loading...
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
International journal of environmental research and public health
Abstract
Non-typhoidal Salmonella (NTS) are foodborne pathogens of global public health
significance. The aim of this study was to subtype a collection of 85 NTS originating from poultry
farms in Uganda, and to evaluate a subgroup of phenotypically resistant isolates for common
antimicrobial resistance genes and associated integrons. All isolates were subtyped by pulsed-field
gel electrophoresis (PFGE). Phenotypically resistant isolates (n = 54) were screened by PCR for the
most relevant AMR genes corresponding to their phenotypic resistance pattern, and all 54 isolates
were screened by PCR for the presence of integron class 1 and 2 encoding genes. These genes
are known to commonly encode resistance to ampicillin, tetracycline, ciprofloxacin, trimethoprim,
sulfonamide and chloramphenicol. PFGE revealed 15 pulsotypes representing 11 serotypes from
75 isolates, as 10 were non-typable. Thirty one (57.4%) of the 54 resistant isolates carried at least one
of the seven genes (blaTEM-1, cmlA, tetA, qnrS, sul1, dhfrI, dhfrVII) identified by PCR and six (11%)
carried class 1 integrons. This study has shown that a diversity of NTS-clones are present in Ugandan
poultry farm settings, while at the same time similar NTS-clones occur in different farms and areas.
The presence of resistance genes to important antimicrobials used in human and veterinary medicine
has been demonstrated, hence the need to strengthen strategies to combat antimicrobial resistance at
all levels.
Description
Keywords
Antimicrobial resistance, Genotypes, Non-typhoidal Salmonella, Poultry, Genes, Integrons, Subtyping
Citation
Odoch, T., Sekse, C., L’Abee-Lund, T. M., Høgberg Hansen, H. C., Kankya, C., & Wasteson, Y. (2018). Diversity and antimicrobial resistance genotypes in non-typhoidal Salmonella isolates from poultry farms in Uganda. International journal of environmental research and public health, 15(2), 324. doi:10.3390/ijerph15020324